Use of Human Hybridoma Technology To Isolate Human Monoclonal Antibodies.
Use of Human Hybridoma Technology To Isolate Human Monoclonal Antibodies.
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DOI：
10.1128/microbiolspec.aid-0027-2014
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发表时间：
2015-03
影响因子：
3.7
通讯作者：
Crowe JE Jr
中科院分区：
文献类型：
--
作者：
Smith SA;Crowe JE Jr
人杂交瘤技术为分离人源性单抗提供了一种重要的方法。可以使用不同的方法，取得不同的成功。最近在扩大人类抗原特异性B细胞的起始数量、提高融合效率、分离新的骨髓瘤伙伴和新的细胞克隆方法方面的技术进步使从血液样本中分离人类单抗成为可能。毫无疑问，可以提高效率的其他创新是可能的。
The human hybridoma technique offers an important approach for isolation of human monoclonal antibodies. A diversity of approaches can be used with varying success. Recent technical advances in expanding the starting number of human antigen-specific B cells, improving fusion efficiency, and isolating new myeloma partners and new cell cloning methods have enabled the development of protocols that make the isolation of human monoclonal antibodies from blood samples feasible. Undoubtedly, additional innovations that could improve efficiency are possible.
影响因子：
8.8
作者：
Sikora, K;Alderson, T;Ellis, J;Phillips, J;Watson, J
通讯作者：
Watson, J