The organization of engaged and quiescent translocons in the endoplasmic reticulum of mammalian cells.
The organization of engaged and quiescent translocons in the endoplasmic reticulum of mammalian cells.
复制标题
DOI：
10.1083/jcb.200312079
复制
发表时间：
2004-03-29
期刊：
影响因子：
--
通讯作者：
Hegde RS
中科院分区：
文献类型：
--
作者：
Snapp EL;Reinhart GA;Bogert BA;Lippincott-Schwartz J;Hegde RS
哺乳动物内质网的蛋白质转座子由许多功能组分组成，其在体内运输周期的不同阶段的组织仍然知之甚少。我们已经开发了基于荧光共振能量转移（FRET）的普遍适用的方法来探测细胞中内源性表达的易位子组分的相对接近性。检查基板从事translocons揭示低聚组件的Sec61复杂的相关程度不同的其他基本组件，包括信号识别颗粒受体TRAM和TRAP复合物。值得注意的是，这些组件不仅保持组装，但也有一个类似的，但可区分的，组织期间和之后的新生链易位。在连续的运输轮之间的预组装和完整的translocons的持久性可以促进体内高效的易位，尽管由正在进行的翻译和拥挤的细胞环境所施加的时间限制。
Protein translocons of the mammalian endoplasmic reticulum are composed of numerous functional components whose organization during different stages of the transport cycle in vivo remains poorly understood. We have developed generally applicable methods based on fluorescence resonance energy transfer (FRET) to probe the relative proximities of endogenously expressed translocon components in cells. Examination of substrate-engaged translocons revealed oligomeric assemblies of the Sec61 complex that were associated to varying degrees with other essential components including the signal recognition particle receptor TRAM and the TRAP complex. Remarkably, these components not only remained assembled but also had a similar, yet distinguishable, organization both during and after nascent chain translocation. The persistence of preassembled and complete translocons between successive rounds of transport may facilitate highly efficient translocation in vivo despite temporal constraints imposed by ongoing translation and a crowded cellular environment.
登录
查看更多内容
DOI：
10.1083/jcb.142.1.69
发表时间：
1998-07-13
期刊：
The Journal of cell biology
影响因子：
--
作者：
Kenworthy AK;Edidin M
通讯作者：
Edidin M
影响因子：
5.6
作者：
Morgan, DG;Ménétret, JF;Akey, CW
通讯作者：
Akey, CW
影响因子：
64.5
作者：
GORLICH, D;RAPOPORT, TA
通讯作者：
RAPOPORT, TA
影响因子：
64.5
作者：
JUNGNICKEL, B;RAPOPORT, TA
通讯作者：
RAPOPORT, TA
在蛋白质靶向内质网期间，SRP 受体对核糖体和信号识别颗粒的双重识别。
DOI：
10.1083/jcb.200303143
发表时间：
2003-08-18
期刊：
The Journal of cell biology
影响因子：
--
作者：
Mandon EC;Jiang Y;Gilmore R
通讯作者：
Gilmore R