Regulation of Arabidopsis photoreceptor CRY2 by two distinct E3 ubiquitin ligases.
Regulation of Arabidopsis photoreceptor CRY2 by two distinct E3 ubiquitin ligases.
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DOI：
10.1038/s41467-021-22410-x
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发表时间：
2021-04-12
影响因子：
16.6
通讯作者：
Lin C
中科院分区：
文献类型：
--
作者：
Chen Y;Hu X;Liu S;Su T;Huang H;Ren H;Gao Z;Wang X;Lin D;Wohlschlegel JA;Wang Q;Lin C
隐色素（CRYs）是各种进化谱系中的光感受器或分子钟的组成部分，通常受多泛素化和蛋白水解的调节。多种E3泛素连接酶在动物模型中调控CRYs，既往遗传学研究也提示存在多种E3泛素连接酶调控植物CRYs。然而，到目前为止，只有一种E3连接酶Cul4COP1/ spa被报道用于植物CRYs。我们发现Cul3LRBs是拟南芥中CRY2的第二个E3连接酶。我们证明了LRBs （Light-Response brick -a- brack /Tramtrack/Broad 1、2和3）在蓝光调控下胚轴伸长中的蓝光特异性和cry依赖性活性。LRBs在CRY2的CCE结构域与光激发和磷酸化的CRY2发生物理相互作用，促进CRY2在蓝光下的多泛素化和降解。我们提出Cul4COP1/ spa和Cul3LRBs E3连接酶通过不同的结构元件与CRY2相互作用，调节CRY2光受体在不同光照条件下的丰度，促进自然生长植物的最佳光响应。细胞中蛋白质的命运不仅由合成决定，也由降解决定。Chen等研究表明，植物蓝光受体CRY2的降解是由两种不同的E3泛素连接酶Cul4COP1/ spa和Cul3LRBs决定的，它们在不同的光照条件下调节CRY2的功能。
Cryptochromes (CRYs) are photoreceptors or components of the molecular clock in various evolutionary lineages, and they are commonly regulated by polyubiquitination and proteolysis. Multiple E3 ubiquitin ligases regulate CRYs in animal models, and previous genetics study also suggest existence of multiple E3 ubiquitin ligases for plant CRYs. However, only one E3 ligase, Cul4COP1/SPAs, has been reported for plant CRYs so far. Here we show that Cul3LRBs is the second E3 ligase of CRY2 in Arabidopsis. We demonstrate the blue light-specific and CRY-dependent activity of LRBs (Light-Response Bric-a-Brack/Tramtrack/Broad 1, 2 & 3) in blue-light regulation of hypocotyl elongation. LRBs physically interact with photoexcited and phosphorylated CRY2, at the CCE domain of CRY2, to facilitate polyubiquitination and degradation of CRY2 in response to blue light. We propose that Cul4COP1/SPAs and Cul3LRBs E3 ligases interact with CRY2 via different structure elements to regulate the abundance of CRY2 photoreceptor under different light conditions, facilitating optimal photoresponses of plants grown in nature. The fate of proteins in cells is determined by not only synthesis but also degradation. Here Chen et al. show that degradation of the plant blue light receptor CRY2 is determined by two distinct E3 ubiquitin ligases, Cul4COP1/SPAs and Cul3LRBs, regulating the function of CRY2 under different light conditions.
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影响因子：
16.6
作者：
Ni W;Xu SL;González-Grandío E;Chalkley RJ;Huhmer AFR;Burlingame AL;Wang ZY;Quail PH
通讯作者：
Quail PH
拟南芥隐花色素2蓝光依赖性磷酸化的分子基础
影响因子：
16.6
作者：
Liu Q;Wang Q;Deng W;Wang X;Piao M;Cai D;Li Y;Barshop WD;Yu X;Zhou T;Liu B;Oka Y;Wohlschlegel J;Zuo Z;Lin C
通讯作者：
Lin C
DOI：
10.1126/science.1250778
发表时间：
2014-06-06
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Ni W;Xu SL;Tepperman JM;Stanley DJ;Maltby DA;Gross JD;Burlingame AL;Wang ZY;Quail PH
通讯作者：
Quail PH
影响因子：
18
作者：
Ban Z;Estelle M
通讯作者：
Estelle M
DOI：
10.1073/pnas.95.5.2686
发表时间：
1998-03-03
影响因子：
11.1
作者：
Lin, CT;Yang, HY;Cashmore, AR
通讯作者：
Cashmore, AR