Liposomal Delivery of MicroRNA-7 Targeting EGFR to Inhibit the Growth, Invasion, and Migration of Ovarian Cancer.
Liposomal Delivery of MicroRNA-7 Targeting EGFR to Inhibit the Growth, Invasion, and Migration of Ovarian Cancer.
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DOI：
10.1021/acsomega.1c00992
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发表时间：
2021-05-04
期刊：
影响因子：
4.1
通讯作者：
Di W
中科院分区：
文献类型：
--
作者：
Cui X;Song K;Lu X;Feng W;Di W
卵巢癌侵袭性强，复发率和转移率高。由于目前治疗的效果有限，有必要进行研究并开发新的治疗方案。基因治疗在肿瘤治疗中的应用逐渐增多，具有令人振奋的前景。MicroRNA-7(miR-7)可抑制多种实体肿瘤的生长、侵袭和转移。阳离子脂质体是一种安全有效的体内、外基因转导系统。为了解miR-7在卵巢癌治疗中的应用，以1，2-二油酰基-3-三甲基丙烷、1，2-dioleoyl-sn-glycero-3-phosphoethanolamine，和胆固醇为原料，制备了阳离子脂质体。MiR-7脂质体具有合适的颗粒大小、潜力和较高的细胞摄取率。脂质体包裹的miR-7可以有效地输送到卵巢癌细胞，并在小鼠卵巢癌异种移植模型中成功地靶向肿瘤部位。体外和体内实验表明，miR-7脂质体具有显著的抑制卵巢癌生长、侵袭和转移的能力，可能是通过抑制表皮生长因子受体的表达而实现的。我们对miR-7脂质体的研究证明了一种安全有效的用于卵巢癌基因治疗的microRNA递送系统。
Ovarian cancer is highly aggressive and has high rates of recurrence and metastasis. Due to the limited effects of current treatments, it is necessary to conduct research and develop new treatment options. The application of gene therapy in tumor therapy is gradually increasing and has exciting prospects. MicroRNA-7 (miR-7) has been reported to inhibit the growth, invasion, and metastasis of a variety of solid tumors. Cationic liposomes are safe and effective gene delivery systems for transfection in vivo and in vitro. To realize the application of miR-7 in the treatment of ovarian cancer, cationic liposomes were prepared with 1,2-dioleoyl-3-trimethylammonium-propane, 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine, and cholesterol. The miR-7 liposomes had a suitable particle size, potential, and a high cellular uptake rate. MiR-7 encapsulated by liposomes could be effectively delivered to ovarian cancer cells and successfully targeted to the tumor site in a mouse xenograft model of ovarian cancer. In vitro and in vivo experiments revealed that the miR-7 liposomes had a significant ability to inhibit the growth, invasion, and migration of ovarian cancer, probably by inhibiting the expression of the epidermal growth factor receptor. Our studies of miR-7 liposomes demonstrated a safe and efficient microRNA delivery system for the gene therapy of ovarian cancer.
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影响因子：
14
作者：
Ambardekar, Vishakha V.;Han, Huai-Yun;Varney, Michelle L.;Vinogradov, Serguei V.;Singh, Rakesh K.;Vetro, Joseph A.
通讯作者：
Vetro, Joseph A.
影响因子：
--
作者：
Amreddy N;Babu A;Muralidharan R;Panneerselvam J;Srivastava A;Ahmed R;Mehta M;Munshi A;Ramesh R
通讯作者：
Ramesh R
影响因子：
16.6
作者：
Julin, Sofia;Nonappa;Kostiainen, Mauri A.
通讯作者：
Kostiainen, Mauri A.
E3 泛素连接酶 NEDD4 介导肺癌细胞中 EGFR 的细胞迁移信号。
影响因子：
37.3
作者：
Shao G;Wang R;Sun A;Wei J;Peng K;Dai Q;Yang W;Lin Q
通讯作者：
Lin Q
影响因子：
3.4
作者：
Kim, Bieong-Kil;Hwang, Guen-Bae;Doh, Kyung-Oh
通讯作者：
Doh, Kyung-Oh