Preparation of DOPC and DPPC Supported Planar Lipid Bilayers for Atomic Force Microscopy and Atomic Force Spectroscopy.
Preparation of DOPC and DPPC Supported Planar Lipid Bilayers for Atomic Force Microscopy and Atomic Force Spectroscopy.
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DOPC和DPPC的制备支持原子力显微镜和原子力光谱的平面脂质双层。
DOI：
10.3390/ijms14023514
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发表时间：
2013-02-06
影响因子：
5.6
通讯作者：
Leonenko Z
中科院分区：
文献类型：
--
作者：
Attwood SJ;Choi Y;Leonenko Z
细胞膜通常非常复杂，由多种不同的脂质和蛋白质组成。支撑的脂质双层被广泛用作研究生物膜的模型系统。原子力显微镜和力光谱技术是纳米尺度的方法，成功地用于研究支持的脂质双层。这些方法，特别是力谱，需要可靠的制备支持的脂质双层与扩展的覆盖范围。然而，对囊泡融合过程的不可靠性和缺乏完整的理解阻碍了这一有前途的领域的进展。我们在这里记录了在云母上形成流体相DOPC和凝胶相DPPC双层的稳健方案。洞察到最关键的实验参数和DOPC和DPPC制备之间的比较。最后，我们证明DOPC表面上的力谱测量和测量破裂力和双层深度，以及同意与X射线衍射数据。我们还相信，我们的方法分解成深度子组件的力-距离曲线提供了一个更可靠的方法来表征流体相脂质双层的深度，特别是在与典型的图像分析方法相比。
Cell membranes are typically very complex, consisting of a multitude of different lipids and proteins. Supported lipid bilayers are widely used as model systems to study biological membranes. Atomic force microscopy and force spectroscopy techniques are nanoscale methods that are successfully used to study supported lipid bilayers. These methods, especially force spectroscopy, require the reliable preparation of supported lipid bilayers with extended coverage. The unreliability and a lack of a complete understanding of the vesicle fusion process though have held back progress in this promising field. We document here robust protocols for the formation of fluid phase DOPC and gel phase DPPC bilayers on mica. Insights into the most crucial experimental parameters and a comparison between DOPC and DPPC preparation are presented. Finally, we demonstrate force spectroscopy measurements on DOPC surfaces and measure rupture forces and bilayer depths that agree well with X-ray diffraction data. We also believe our approach to decomposing the force-distance curves into depth sub-components provides a more reliable method for characterising the depth of fluid phase lipid bilayers, particularly in comparison with typical image analysis approaches.
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影响因子：
5.8
作者：
Dekkiche, F.;Corneci, M. C.;Rieu, J. -P.
通讯作者：
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影响因子：
3.4
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Garcia-Saez, Ana J.;Chiantia, Salvatore;Schwille, Petra
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通讯作者：
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通讯作者：
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