Producing fast and active Rubisco in tobacco to enhance photosynthesis.
Producing fast and active Rubisco in tobacco to enhance photosynthesis.
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在烟草中培育快速且活性高的核酮糖 - 1,5 - 二磷酸羧化酶/加氧酶（Rubisco）以增强光合作用。
DOI：
10.1093/plcell/koac348
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发表时间：
2023-02-20
期刊：
影响因子：
11.6
通讯作者：
Liu, Lu-Ning
中科院分区：
文献类型：
--
作者：
Chen, Taiyu;Riaz, Saba;Davey, Philip;Zhao, Ziyu;Sun, Yaqi;Dykes, Gregory F.;Zhou, Fei;Hartwell, James;Lawson, Tracy;Nixon, Peter J.;Lin, Yongjun;Liu, Lu-Ning
核酮糖-1,5-二磷酸羧化酶/加氧酶（Rubisco）完成了地球上大部分的碳固定。然而，由于其低羧化率，植物Rubisco本质上是一种效率低下的酶，这是光合作用的主要限制。用更快的Rubisco取代内源植物Rubisco有望提高作物的光合作用和生产力。然而，Rubisco在叶绿体中的表达和组装对伴侣的要求阻碍了功能性外源Rubisco在叶绿体中的高效生产。本文报道了从大肠杆菌和烟草（Nicotiana tabacum）叶绿体中的新波利盐硫杆菌（Halothiobacillus neapolitanus）中提取的1A型Rubisco的工程研究。将原烟草编码Rubisco大亚基的基因遗传替换为H. neapolitanus Rubisco （HnRubisco）大亚基和小亚基基因。我们发现HnRubisco亚基可以在烟草叶绿体中高效率地形成功能性的L8S8 16位体，占野生型烟草Rubisco含量的约40%。叶绿体表达的HnRubisco显示出高2倍的羧化速率，并支持转基因植物在添加1% CO2的空气中与野生型相似的自养生长速率。该研究为在叶绿体中构建快速、高活性的Rubisco以改善作物光合作用和生长迈出了一步。在烟草叶绿体中引入具有较高羧化率和较高活性位点含量的Rubisco，可支持高CO2浓度下的光合作用和生长。
Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) performs most of the carbon fixation on Earth. However, plant Rubisco is an intrinsically inefficient enzyme given its low carboxylation rate, representing a major limitation to photosynthesis. Replacing endogenous plant Rubisco with a faster Rubisco is anticipated to enhance crop photosynthesis and productivity. However, the requirement of chaperones for Rubisco expression and assembly has obstructed the efficient production of functional foreign Rubisco in chloroplasts. Here, we report the engineering of a Form 1A Rubisco from the proteobacterium Halothiobacillus neapolitanus in Escherichia coli and tobacco (Nicotiana tabacum) chloroplasts without any cognate chaperones. The native tobacco gene encoding Rubisco large subunit was genetically replaced with H. neapolitanus Rubisco (HnRubisco) large and small subunit genes. We show that HnRubisco subunits can form functional L8S8 hexadecamers in tobacco chloroplasts at high efficiency, accounting for ∼40% of the wild-type tobacco Rubisco content. The chloroplast-expressed HnRubisco displayed a ∼2-fold greater carboxylation rate and supported a similar autotrophic growth rate of transgenic plants to that of wild-type in air supplemented with 1% CO2. This study represents a step toward the engineering of a fast and highly active Rubisco in chloroplasts to improve crop photosynthesis and growth. Introducing a proteobacterial Rubisco with a greater carboxylation rate and a higher content of active sites into tobacco chloroplasts supports photosynthesis and growth at high CO2 concentrations.
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影响因子：
4.7
作者：
Chen T;Fang Y;Jiang Q;Dykes GF;Lin Y;Price GD;Long BM;Liu LN
通讯作者：
Liu LN
影响因子：
64.8
作者：
GATENBY, AA;VANDERVIES, SM;BRADLEY, D
通讯作者：
BRADLEY, D
DOI：
10.1073/pnas.2007990117
发表时间：
2020-07-21
影响因子：
11.1
作者：
Huang, Fang;Kong, Wen-Wen;Liu, Lu-Ning
通讯作者：
Liu, Lu-Ning
影响因子：
7.8
作者：
Erb TJ;Zarzycki J
通讯作者：
Zarzycki J
DOI：
10.1073/pnas.1816654116
发表时间：
2019-03-05
影响因子：
11.1
作者：
Bar-On, Yinon M.;Milo, Ron
通讯作者：
Milo, Ron