OMIP‐090: A 20‐parameter flow cytometry panel for rapid analysis of cell diversity and homing capacity in human conventional and regulatory T cells
OMIP‐090: A 20‐parameter flow cytometry panel for rapid analysis of cell diversity and homing capacity in human conventional and regulatory T cells
复制标题
OMIP-090：20 参数流式细胞术面板，用于快速分析人类常规 T 细胞和调节性 T 细胞的细胞多样性和归巢能力
作者：
Wladislaw Stroukov;D. Mastronicola;C. J. Albany;Zeynep Catak;G. Lombardi;C. Scottà
该仪表板是为监测人类CD4+常规和调节性T细胞亚群归巢能力和功能多样性的变化而开发和优化的。该分析基于新鲜分离的外周血单个核细胞(PBMC)中仅表达表面标志，以最大限度地减少因渗透或冷冻/解冻过程而造成的任何变化。我们根据CD45RA、CCR7、CD25、CD28和CD95在常规T细胞和调节性T细胞中的表达来评估幼稚和记忆群体的分布。使用CCR4、CCR6、CCR10、CXCR3和CXCR5对主要功能亚群进行鉴定。这些亚群对皮肤、呼吸道、肠道和炎性病变的归巢能力最终可以通过标记物Cla、CCR3、CCR5和整合素β7进行评估。该小组在新鲜分离的健康供者和变应性鼻炎或自身免疫性疾病患者的PBMC上进行了测试。
The panel was developed and optimized for monitoring changes in homing capacity and functional diversity of human CD4+ conventional and regulatory T cell subsets. The analysis was based on expression of only surface markers in freshly isolated peripheral blood mononuclear cells (PBMCs) to reduce at minimum any alteration due to permeabilization or freezing/thawing procedures. We included markers to assess the distribution of naïve and memory populations based on the expression of CD45RA, CCR7, CD25, CD28 and CD95 in both conventional and regulatory T cells. The identification of major functional subsets was performed using CCR4, CCR6, CCR10, CXCR3 and CXCR5. Homing capacity of these subsets to skin, airway tract, gut and inflammatory lesions could finally be assessed with the markers CLA, CCR3, CCR5 and integrin β7. The panel was tested on freshly isolated PBMCs from healthy donors and patients with allergic rhinitis or autoimmune disorders.
影响因子：
20.3
作者：
Duhen, Thomas;Duhen, Rebekka;Campbell, Daniel J.
通讯作者：
Campbell, Daniel J.
影响因子：
32.4
作者：
Kerfoot SM;Yaari G;Patel JR;Johnson KL;Gonzalez DG;Kleinstein SH;Haberman AM
通讯作者：
Haberman AM
DOI：
10.1073/pnas.96.12.6873
发表时间：
1999-06-08
影响因子：
11.1
作者：
Balashov, KE;Rottman, JB;Hancock, WW
通讯作者：
Hancock, WW