Light microscopic observations of the ruminal papillae of cattle on diets with divergent forage to cereal ratios.
Light microscopic observations of the ruminal papillae of cattle on diets with divergent forage to cereal ratios.
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DOI：
10.1016/j.animal.2022.100462
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发表时间：
2022-03
期刊：
影响因子：
--
通讯作者：
Jonsson NN
中科院分区：
文献类型：
--
作者：
Ferguson HJ;Koh-Tan HHC;Johnston PEJ;Wallace RJ;Andonovic I;Michie C;McCartney CA;Strachan EM;Snelling TJ;Harvey CD;Thomson W;Jonsson NN
特征性组织学变化与膳食谷物比例相关。在食用较低谷物饮食的牛中发现免疫相关细胞的密度较高。谷物比例与细胞层厚度、完整性和脱落有关。与苏木精和曙红相比，使用 Elastin Martius 猩红蓝染色剂没有任何优势。新颖的评分系统可以通过大致的膳食谷物水平来区分群体。高水平补充谷物可以提高牛的生产率，但也会增加疾病的发生率，包括轻度消化不良到急性瘤胃酸中毒和死亡。因此，有动机确定可用于识别动物是否已经或正在被喂食充足或过量谷物的生物标记。本研究旨在描述喂食不同膳食谷物比例的动物的光学显微镜观察结果，并测试新型瘤胃上皮评分系统的性能。瘤胃壁组织样本取自屠宰场，来自 11 个苏格兰农场的 195 头牛，并进行处理以进行组织学检查。使用光学显微镜检查来表征瘤胃上皮对饮食挑战的反应。次要目标包括描述牛瘤胃上皮中免疫相关细胞的分布，并评估改良的 Elastin Martius 猩红蓝染色 (EMSB) 在瘤胃上皮组织学检查中的使用。分化簇3染色呈阳性的细胞主要分布在基底层的下层，并且在提供较低谷物比例饮食的动物中发现密度较高。主要组织相容性复合体 2 类 (MHCII) 染色呈阳性的细胞最常见于血管周围位置以及下层基底层和固有粘膜下层之间的连接处。低谷类饮食动物的 MHCII 阳性染色细胞密度较高。谷物的补充水平还与角质层（SCT）和颗粒层（SGT）的厚度、角质层的完整性和角质化细胞的脱落有关。在此评分系统中，使用 EMSB 染色相对于苏木精和伊红 (H&E) 没有优势。我们得出的结论是，仅包括 SCT、SGT 和细胞间隙外观损失测量的评分系统允许根据谷物补充水平区分动物组。
Characteristic histological change is associated with dietary cereal proportion. Immune-related cells are found in higher densities in cattle on lower cereal diets. Cereal proportion is associated with cell layer thickness, integrity, and sloughing. No benefit of Elastin Martius Scarlet Blue stain use over Haematoxylin and Eosin. Novel scoring system can differentiate groups by approximate dietary cereal level. High levels of supplementation with cereal increases production rates in cattle but can increase incidence of disease, ranging from mild indigestion to acute ruminal acidosis and death. Therefore, there is motivation to determine biological markers which can be used to identify whether animals have been, or are being fed, sufficient or excessive cereals. This study aimed to describe light microscopic findings from animals being fed diverse dietary cereal proportions and to test the performance of a novel rumen epithelial scoring system. Rumen wall tissue samples were obtained from the abattoir from 195 cattle from 11 Scottish farms and processed for histological examination. Light microscopic examination was used to characterise ruminal epithelial response to dietary challenge. Secondary objectives included describing the distribution of immune-related cells in bovine ruminal epithelium and assessing the use of a modified Elastin Martius Scarlet Blue stain (EMSB) for histological examination of the rumen epithelium. Cells staining positive for cluster of differentiation 3 were distributed mainly in the lower layers of the stratum basale and were found in higher densities in animals offered lower cereal proportion diets. Cells staining positive for major histocompatibility complex class 2 (MHCII) were most common in perivascular locations and in the junction between the lower stratum basale and the propria-submucosa. The density of MHCII positive staining cells was higher in animals on lower cereal diets. The level of supplementation with cereal was also associated with the thickness of the stratum corneum (SCT) and stratum granulosum (SGT), the integrity of the stratum corneum and sloughing of cornified cells. There were no advantages in using EMSB stain over haematoxylin and eosin (H&E) in this scoring system. We concluded that a scoring system that included only SCT, SGT and a measure of the loss of appearance of intercellular space allowed differentiation of groups of animals according to the level of cereal supplementation.
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影响因子：
3.3
作者：
Metzler-Zebeli, B. U.;Hollmann, M.;Zebeli, Q.
通讯作者：
Zebeli, Q.
影响因子：
3.5
作者：
Emmanuel, D. G. V.;Dunn, S. M.;Ametaj, B. N.
通讯作者：
Ametaj, B. N.
影响因子：
3.5
作者：
Gozho, G. N.;Krause, D. O.;Plaizier, J. C.
通讯作者：
Plaizier, J. C.
DOI：
10.1152/ajpgi.00304.2010
发表时间：
2011-08-01
影响因子：
4.5
作者：
Bondzio, Angelika;Gabler, Christoph;Einspanier, Ralf
通讯作者：
Einspanier, Ralf
影响因子：
4.2
作者：
Shen, ZM;Seyfert, HM;Voigt, J
通讯作者：
Voigt, J