Hormone-induced ductal DNA synthesis of human breast tissues maintained in the athymic nude mouse.
Hormone-induced ductal DNA synthesis of human breast tissues maintained in the athymic nude mouse.
复制标题
无胸腺裸鼠体内激素诱导的人类乳腺组织导管 DNA 合成。
作者：
M. Mcmanus;C. Welsch
5例形态正常的人乳腺组织标本，取自5例乳腺良性肿瘤的边缘组织，切片(4.0×4.0×0.1 mm)，移植皮下。背部(8~10片/只)取6~8周龄雌性BALB/c裸鼠43只。每个人的乳房组织样本都被移植到7到10只小鼠体内。30天后，将小鼠分成4组，治疗30天如下：(1)对照组，皮下注射。胆固醇颗粒(38毫克)；(B)南卡罗来纳州给予的雌激素。(C)大鼠脑垂体瘤(RPT)，一种可分泌大量催乳素和生长激素的MTTW10RPT细胞悬液，经背部注射；(D)RPT加雌激素。在治疗开始时，从每只小鼠身上取出三到五个人乳房组织移植物，其余的在治疗结束时被取出。用[~3H]胸腺嘧啶核苷放射自显影技术测定处理前后的移植物在富含同位素的培养液中孵育4小时后导管上皮细胞的DNA合成。标记指数(LI)分别为：(A)对照组，7.6+/-1.4和7.1+/-1.4，(B)雌激素，5.5+/-0.6和17.9+/-2.3，(C)RPT，6.2+/-0.7和8.0+/-1.5，(C)RPT，6.2+/-0.7和8.0+/-1.5；和(D)RPT加雌激素，6.3+/-0.8和26.6+/-2.5。经雌激素治疗(P<0.001)或雷公藤多糖加雌激素治疗后，LI显著升高(P<0.05)。RPT加雌激素治疗后的平均LI明显大于单用雌激素治疗后的LI(p<0.02)。RPT本身并没有显著改变LI。因此，这些结果提供了体内证据，证明雌激素促进正常人乳腺导管上皮细胞的DNA合成，并且来自RPT的生长因子(或多个因子)与雌激素协同作用，产生更显著的DNA合成增加。RPT生长因子(可能是催乳素和/或生长激素)似乎需要雌激素来刺激正常人类乳腺导管上皮的DNA合成。
Five biopsy specimens of morphologically normal human breast tissues, obtained from the margins of five benign human breast tumors, were processed into slices (4.0 x 4.0 x 0.1 mm) and transplanted s.c. dorsally (eight to ten slices/mouse) into forty-three 6- to 8-week-old female BALB/c athymic nude mice. Each individual human breast tissue specimen was transplanted into seven to ten mice. After 30 days, the mice were divided into four groups and treated for 30 days as follows: (a) controls receiving s.c. cholesterol pellets (38 mg); (b) estrogens that were administered in s.c. pellets containing 2 mg 17 beta-estradiol and 38 mg cholesterol and in drinking water containing 0.5 mg estrone per liter; (c) rat pituitary tumor (RPT), a cell suspension of MtT-W10 RPT that secretes large amounts of prolactin and growth hormone, injected dorsorostrally; and (d) RPT plus estrogens. Three to five human breast tissue grafts were removed from each mouse at the onset of treatment, and the remainder were removed at termination of treatment. DNA synthesis in the ductal epithelium was determined in pre- and posttreatment grafts by [3H]thymidine autoradiography after incubation of grafts for 4 hr in an isotope-enriched medium. The labeling index (LI), mean number of labeled epithelial cells per unit area of epithelial tissue, in pre- and posttreatment grafts was, respectively: (a) control, 7.6 +/- 1.4 and 7.1 +/- 1.4; (b) estrogens, 5.5 +/- 0.6 and 17.9 +/- 2.3; (c) RPT, 6.2 +/- 0.7 and 8.0 +/- 1.5; and (d) RPT plus estrogens, 6.3 +/- 0.8 and 26.6 +/- 2.5. A significant increase in LI was observed after treatment with estrogens (p less than 0.05) or RPT plus estrogen (p less than 0.001). Mean LI after treatment with RPT plus estrogens was significantly greater (p less than 0.02) than after estrogens alone. RPT alone did not significantly alter the LI. Thus, these results provide in vivo evidence that estrogens enhance DNA synthesis of the ductal epithelium of the normal human breast and that a growth factor (or factors) from RPT acts synergistically with estrogens to produce a more pronounced increase in DNA synthesis. RPT growth factors (perhaps prolactin and/or growth hormone) appear to require estrogens for DNA synthesis stimulation in normal human breast ductal epithelium.
影响因子：
11.2
作者：
R. Burke;S. Harris;W. McGuire
通讯作者：
R. Burke;S. Harris;W. McGuire