Site-specific integration of Streptomyces PhiC31 integrase-based vectors in the chromosome of Rhodococcus equi.
Site-specific integration of Streptomyces PhiC31 integrase-based vectors in the chromosome of Rhodococcus equi.
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DOI：
10.1111/j.1574-6968.2008.01298.x
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发表时间：
2008-10
影响因子：
2.1
通讯作者：
Hondalus MK
中科院分区：
文献类型：
--
作者：
Hong Y;Hondalus MK
Streptomyces ΦC31-based位点特异性整合被用于转化兼性细胞内病原体马红球菌（Rhodococcus equi）。结合ΦC31整合酶和attP位点的载体的转化效率与使用相同电穿孔程序的复制质粒相当。在一个编码pirin样蛋白的ORF中发现了一个单一的attB整合位点，这与链霉菌attB位点的共识序列略有偏离。在体外非选择传代过程中，载体整合在马鼠染色体上稳定地维持了100代。此外，整合似乎并不影响巨噬细胞内细菌的复制。最后，该整合系统也被成功地用于补足一个马蹄草突变体。
Streptomyces ΦC31-based site-specific integration was used to transform the facultative intracellular pathogen Rhodococcus equi. The transformation efficiency of vectors incorporating the ΦC31 integrase and attP sites was comparable to that of replication plasmids using the same electroporation procedure. A single attB integration site was identified within an ORF encoding a pirin-like protein, which deviates slightly from the consensus sequence of Streptomyces attB sites. Vector integration was stably maintained in the R. equi chromosome for as many as 100 generations during unselected passage in vitro. In addition, integration does not appear to affect the replication of bacteria inside macrophages. Finally, this integration system was also used to successfully complement an R. equi mutant.
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DOI：
10.1073/pnas.95.10.5505
发表时间：
1998-05-12
影响因子：
11.1
作者：
Thorpe, HM;Smith, MCM
通讯作者：
Smith, MCM
影响因子：
2.1
作者：
Mangan, MW;Meijer, WG
通讯作者：
Meijer, WG
影响因子：
3.2
作者：
Soo, Po-Chi;Horng, Yu-Tze;Lai, Hsin-Chih
通讯作者：
Lai, Hsin-Chih
影响因子：
3.1
作者：
Wall, DM;Duffy, PS;Meijer, WG
通讯作者：
Meijer, WG
影响因子：
3.1
作者：
TAKAI, S;SEKIZAKI, T;TSUBAKI, S
通讯作者：
TSUBAKI, S