In vitro digestion of galactolipids from chloroplast-rich fraction (CRF) of postharvest, pea vine field residue (haulm) and spinach leaves.
In vitro digestion of galactolipids from chloroplast-rich fraction (CRF) of postharvest, pea vine field residue (haulm) and spinach leaves.
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采后、豌豆田残渣（茎）和菠菜叶富含叶绿体部分 (CRF) 的半乳糖脂的体外消化。
DOI：
10.1039/c9fo01867k
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发表时间：
2019
期刊：
影响因子：
6.1
通讯作者：
Wattanakul J
中科院分区：
文献类型：
--
作者：
Wattanakul J
从细胞壁中去除完整的叶绿体，为从绿色生物质中获取亲脂性营养物质，特别是类胡萝卜素和半乳糖脂提供了一种新的途径。后者是植物的主要膜脂，它们是必需α-亚麻酸的主要来源（18:3；ALA）。然而，关于它们消化的知识仍然有限。我们开发了一种从绿色生物质中回收富含叶绿体的组分（CRF）的物理方法，并在模拟胃肠道条件下测试了其体外消化率。采用两步静态模型，首先将菠菜叶和采后豌豆藤田残渣的CRF暴露于兔胃提取物的酶中，然后暴露于人胰液（HPJ）或猪胰腺提取物（PPE）的胰腺酶中。采用脂肪酸甲酯薄层色谱和气相色谱法对单半乳糖二酰基甘油（MGDG）和双半乳糖二酰基甘油（DGDG）的脂解过程进行了监测。两种CRF制剂中，MGDG和DGDG在肠期分别转化为单半乳糖单酰基甘油（MGMG）和双半乳糖单酰基甘油（DGMG）， ALA是主要释放的脂肪酸。HPJ比PPE更有效地水解半乳糖脂，PPE对MGDG的活性高于DGDG。这些发现可能是由于HPJ中半乳糖脂肪酶活性高于PPE，这主要是由胰腺脂肪酶相关蛋白2引起的。因此，我们发现CRF半乳糖脂能被胰腺酶很好地消化，是人类饮食中补充ALA的一种有趣的载体。
The removal of intact chloroplasts from their cell wall confinement offers a novel way to obtain lipophilic nutrients from green biomass, especially carotenoids and galactolipids. These latter are the main membrane lipids in plants and they represent a major source of the essential α-linolenic acid (18:3; ALA). Nevertheless, knowledge on their digestion is still limited. We have developed a physical method of recovering a chloroplast-rich fraction (CRF) from green biomass and tested its digestibility in vitro under simulated gastrointestinal conditions. Using a two-step static model, CRF from both spinach leaves and postharvest, pea vine field residue (haulm) were first exposed to enzymes from rabbit gastric extracts and then either to pancreatic enzymes from human pancreatic juice (HPJ) or to porcine pancreatic extracts (PPE). The lipolysis of monogalactosyldiacylglycerol (MGDG) and digalactosyl diacylglycerol (DGDG) was monitored by thin layer chromatography and gas chromatography of fatty acid methyl esters. For both CRF preparations, MGDG and DGDG were converted to monogalactosylmonoacylglycerol (MGMG) and digalactosylmonoacylglycerol (DGMG), respectively, during the intestinal phase and ALA was the main fatty acid released. Galactolipids were more effectively hydrolysed by HPJ than by PPE, and PPE showed a higher activity on MGDG than on DGDG. These findings may be explained by the higher levels of galactolipase activity in HPJ compared to PPE, which mainly results from pancreatic lipase-related protein 2. Thus, we showed that CRF galactolipids are well digested by pancreatic enzymes and represent an interesting vehicle for ALA supplementation in human diet.
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人胰腺脂肪分解酶和十二指肠内容物水解半乳糖脂。
影响因子：
6.5
作者：
L. Andersson;C. Bratt;K. Arnoldsson;B. Herslöf;N. U. Olsson;B. Sternby;A. Nilsson
通讯作者：
A. Nilsson
DOI：
10.1016/j.bbalip.2009.05.002
发表时间：
2009-10-01
影响因子：
4.8
作者：
Amara, Sawsan;Lafont, Dominique;De Caro, Alain
通讯作者：
De Caro, Alain
影响因子：
3.1
作者：
Gedi, Mohamed A.;Briars, Rhianna;Gray, David A.
通讯作者：
Gray, David A.
半乳糖脂作为植物食品中潜在的健康促进化合物。
影响因子：
--
作者：
L. Christensen
通讯作者：
L. Christensen
来自豌豆 (Pisum sativum) 采后田间残留物 (Haulm) 物理分馏的富含叶绿体的材料。
影响因子：
8.8
作者：
Torcello-Gómez A
通讯作者：
Torcello-Gómez A