Decrease in thyroid adenoma associated (THADA) expression is a marker of dedifferentiation of thyroid tissue.
Decrease in thyroid adenoma associated (THADA) expression is a marker of dedifferentiation of thyroid tissue.
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DOI：
10.1186/1472-6890-11-13
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发表时间：
2011-11-04
影响因子：
--
通讯作者：
Bullerdiek J
中科院分区：
文献类型：
--
作者：
Kloth L;Belge G;Burchardt K;Loeschke S;Wosniok W;Fu X;Nimzyk R;Mohamed SA;Drieschner N;Rippe V;Bullerdiek J
甲状腺腺瘤相关基因（THADA）已被确定为甲状腺腺瘤中染色体2p21易位影响的靶基因，但THADA在甲状腺中的作用仍然难以捉摸。本研究的目的是定量THADA基因表达在正常组织和甲状腺增生和肿瘤，使用实时PCR。为了分析THADA和18S rRNA基因表达测定进行了34个正常组织样本，包括甲状腺，唾液腺，心脏，子宫内膜，子宫肌层，肺，血液和脂肪组织以及85甲状腺增生和肿瘤，包括3个腺瘤与2p21易位。此外，NIS（钠碘同向转运体）基因的表达进行了测量34病理甲状腺样本。结果表明，正常甲状腺组织中的THADA表达显著高于其他组织（p <0.0001，精确Wilcoxon检验）。在非恶性病理甲状腺样品（甲状腺肿和腺瘤）与恶性肿瘤（p <0.001，Wilcoxon检验，t近似）、间变性癌（ATC）与所有其他样品之间以及ATC与所有其他恶性肿瘤之间也发现了显著差异（p <0.05，Wilcoxon检验，t近似）。此外，在甲状腺肿瘤中，发现THADA mRNA表达与HMGA 2 mRNA呈负相关。HMGA 2表达最近被确定为揭示甲状腺滤泡性肿瘤恶性转化的标志物。在甲状腺正常组织和恶性肿瘤中也发现了THADA和NIS之间的相关性。提示THADA可作为甲状腺组织去分化的标志物。
Thyroid adenoma associated (THADA) has been identified as the target gene affected by chromosome 2p21 translocations in thyroid adenomas, but the role of THADA in the thyroid is still elusive. The aim of this study was to quantify THADA gene expression in normal tissues and in thyroid hyper- and neoplasias, using real-time PCR. For the analysis THADA and 18S rRNA gene expression assays were performed on 34 normal tissue samples, including thyroid, salivary gland, heart, endometrium, myometrium, lung, blood, and adipose tissue as well as on 85 thyroid hyper- and neoplasias, including three adenomas with a 2p21 translocation. In addition, NIS (sodium-iodide symporter) gene expression was measured on 34 of the pathological thyroid samples. Results illustrated that THADA expression in normal thyroid tissue was significantly higher (p < 0.0001, exact Wilcoxon test) than in the other tissues. Significant differences were also found between non-malignant pathological thyroid samples (goiters and adenomas) and malignant tumors (p < 0.001, Wilcoxon test, t approximation), anaplastic carcinomas (ATCs) and all other samples and also between ATCs and all other malignant tumors (p < 0.05, Wilcoxon test, t approximation). Furthermore, in thyroid tumors THADA mRNA expression was found to be inversely correlated with HMGA2 mRNA. HMGA2 expression was recently identified as a marker revealing malignant transformation of thyroid follicular tumors. A correlation between THADA and NIS has also been found in thyroid normal tissue and malignant tumors. The results suggest THADA being a marker of dedifferentiation of thyroid tissue.
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影响因子：
5.8
作者：
Bidart, JM;Lacroix, L;Schlumberger, M
通讯作者：
Schlumberger, M
影响因子：
11.5
作者：
Prasad, Nijaguna B.;Somervell, Helina;Zeiger, Martha A.
通讯作者：
Zeiger, Martha A.
影响因子：
5.8
作者：
Arturi, F;Russo, D;Filetti, S
通讯作者：
Filetti, S
DOI：
10.1159/000059344
发表时间：
2001-01-01
期刊：
CYTOGENETICS AND CELL GENETICS
影响因子：
--
作者：
Bol, S;Belge, G;Bullerdiek, J
通讯作者：
Bullerdiek, J
影响因子：
--
作者：
Nguyen, LQ;Kopp, P;Jameson, JL
通讯作者：
Jameson, JL