Design and Application of Piezoelectric Quartz Crystal-based Immunoassay

Design and Application of Piezoelectric Quartz Crystal-based Immunoassay
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压电石英晶体免疫分析的设计与应用

DOI:
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发表时间:
2000
期刊:
影响因子:
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通讯作者:
Sam F. Y. Li
Sam F. Y. Li
中科院分区:
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文献类型:
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作者:
X. Su;F. Chew;Sam F. Y. Li

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本文介绍了压电石英晶体微天平免疫分析的一些基本测试方案。这些方法包括一步直接测定法、一步间接测定法、两步夹心测定法、置换测定法和质量扩增测定法。综述了这些设计在临床诊断、食品分析、环境分析和兽医诊断等领域的应用。此外,还讨论了QCM免疫分析的一些关键问题,包括液相和气相检测的评价、生物分子的包被技术、非特异性吸附和旧晶体的再生。
This paper describes some basic test schemes of piezoelectric quartz crystal microbalance (QCM)-based immunoassay. These include one-step direct assay, one-step indirect assay, two-step sandwiched assay, displacement assay, and mass-amplified assay. Applications of these designs in the fields of clinic diagnosis, food analysis, environmental analysis, and veterinary diagnosis are reviewed. In addition, some critical issues of QCM immunoassay are also dealt with, including the evaluation of liquid phase and gas phase detection, and the biomolecules’ coating techniques, non-specific adsorption, and regeneration of used crystals.
DOI: 10.1080/00032719508000035
发表时间: 1995-01-01
期刊: ANALYTICAL LETTERS
影响因子: 2
作者:
ATTILI, BS;SULEIMAN, AA
通讯作者: SULEIMAN, AA