Comparative analysis of the conserved functions of Arabidopsis DRL1 and yeast KTI12.
Comparative analysis of the conserved functions of Arabidopsis DRL1 and yeast KTI12.
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DOI：
10.14348/molcells.2015.2297
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发表时间：
2015-03
影响因子：
3.8
通讯作者：
Kim GT
中科院分区：
文献类型：
--
作者：
Jun SE;Cho KH;Hwang JY;Abdel-Fattah W;Hammermeister A;Schaffrath R;Bowman JL;Kim GT
在叶片发育早期，极轴的形成是通过茎尖分生组织（SAM）和叶片原基之间的细胞间交流来建立的。在之前的一项研究中，我们发现DRL1基因编码酵母伸长相关蛋白KTI12的同源物，作为正面叶片模式和茎分生组织活性的正调节因子。为了确定DRL1的进化保守功能，我们对DRL1及其酵母同源物KTI12的推导氨基酸序列进行了比较，发现尽管总体同源性较低，但存在良好的保守结构域。DRL1包含两个保守的植物特异性结构域。在酵母KTI12缺失的酵母突变体中，DRL1基因的表达抑制了生长迟缓表型，但没有恢复咖啡因敏感性，这表明拟南芥伸长相关蛋白的作用在酵母KTI12中部分保守，但在进化过程中，酵母和植物对咖啡因的反应可能发生了变化。此外，DRL1基因的高表达触发了酶素敏感性，而KTI12的过表达维持了酶素抗性，这表明拟南芥DRL1在毒素敏感性方面的功能可能与酵母KTI12不重叠。本研究通过表达分析发现，拟南芥drl1-101突变体茎尖中i类KNOX基因下调，叶片中YAB和KAN基因上调。我们的研究结果揭示了SAM和叶片原基之间的通信网络，通过介导组蛋白乙酰化或通过其他机制来建立叶片极性。
Patterning of the polar axis during the early leaf developmental stage is established by cell-to-cell communication between the shoot apical meristem (SAM) and the leaf primordia. In a previous study, we showed that the DRL1 gene, which encodes a homolog of the Elongator-associated protein KTI12 of yeast, acts as a positive regulator of adaxial leaf patterning and shoot meristem activity. To determine the evolutionally conserved functions of DRL1, we performed a comparison of the deduced amino acid sequence of DRL1 and its yeast homolog, KTI12, and found that while overall homology was low, well-conserved domains were presented. DRL1 contained two conserved plant-specific domains. Expression of the DRL1 gene in a yeast KTI12-deficient yeast mutant suppressed the growth retardation phenotype, but did not rescue the caffeine sensitivity, indicating that the role of Arabidopsis Elongator-associated protein is partially conserved with yeast KTI12, but may have changed between yeast and plants in response to caffeine during the course of evolution. In addition, elevated expression of DRL1 gene triggered zymocin sensitivity, while overexpression of KTI12 maintained zymocin resistance, indicating that the function of Arabidopsis DRL1 may not overlap with yeast KTI12 with regards to toxin sensitivity. In this study, expression analysis showed that class-I KNOX genes were downregulated in the shoot apex, and that YAB and KAN were upregulated in leaves of the Arabidopsis drl1-101 mutant. Our results provide insight into the communication network between the SAM and leaf primordia required for the establishment of leaf polarity by mediating histone acetylation or through other mechanisms.
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