Somatostatin discriminates between the intracellular pathways of secretory and membrane proteins.
Somatostatin discriminates between the intracellular pathways of secretory and membrane proteins.
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DOI：
10.1083/jcb.99.1.97
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发表时间：
1984-07
期刊：
影响因子：
--
通讯作者：
Shields D
中科院分区：
文献类型：
--
作者：
Green R;Shields D
生长抑素是一种由14个氨基酸组成的多肽激素，可抑制包括生长激素在内的多种其他多肽激素的分泌。在这里，我们描述了一个实验系统，用于确定生长抑素是否能够区分其对分泌和质膜蛋白的抑制。用水泡性口炎病毒感染生长激素分泌细胞(GH3)，用[35S]蛋氨酸刺激生长激素分泌细胞，以跟踪生长激素和病毒膜糖蛋白G蛋白在细胞内的同时转运。Chase培养基免疫沉淀法检测生长激素的分泌，细胞表面标记和病毒纯化检测细胞质膜G蛋白的表达。在生长抑素(10微克/毫升)存在下，生长激素的分泌被抑制了80%。相反，G蛋白出现在质膜上，动力学略有增强。当用离子载体莫能菌素(0.2微米)处理细胞时，生长激素的分泌和G蛋白掺入质膜都受到显著的抑制。我们关于生长抑素不同作用的结果为将分泌蛋白和膜蛋白分类到分泌途径中的不同区段提供了证据。数据进一步表明，这种分选事件发生在高尔基复合体的晚期或蛋白质离开细胞器之后。
Somatostatin is a14-amino acid peptide hormone that inhibits the secretion of a variety of other polypeptide hormones, including growth hormone. Here we describe an experimental system used to determine whether somatostatin can discriminate in its inhibition between secretory and plasma membrane proteins. Growth hormone-secreting cells (GH3) were infected with vesicular stomatitis virus and pulse-chased with [35S]methionine to follow the simultaneous intracellular transit of growth hormone and the viral membrane glycoprotein, G protein. Secretion of growth hormone was monitored by immunoprecipitation of chase media, while appearance of G protein on the plasma membrane was detected by cell surface labeling and virus purification. In the presence of somatostatin (10 micrograms/ml), the secretion of growth hormone was inhibited by 80%. In contrast, G protein appeared on the plasma membrane with slightly enhanced kinetics. When cells were treated with the ionophore monensin (0.2 microM), there was a dramatic inhibition of both the secretion of growth hormone and the incorporation of G protein into plasma membranes. Our results on the differential effect of somatostatin provide evidence for sorting of secretory and membrane proteins into distinct compartments in the secretory pathway. The data further suggest that this sorting event occurs late in the Golgi complex or after proteins exit from that organelle.
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DOI：
10.1083/jcb.97.3.810
发表时间：
1983-09
期刊：
The Journal of cell biology
影响因子：
--
作者：
Moore HP;Gumbiner B;Kelly RB
通讯作者：
Kelly RB
影响因子：
64.5
作者：
STROUS, JAM;LODISH, HF
通讯作者：
LODISH, HF
影响因子：
4.8
作者：
BILEZIKJIAN, LM;VALE, WW
通讯作者：
VALE, WW
影响因子：
5.4
作者：
LODISH, HF;PORTER, M
通讯作者：
PORTER, M
影响因子：
5.4
作者：
ROSE, JK;GALLIONE, CJ
通讯作者：
GALLIONE, CJ