Strategies to improve homology-based repair outcomes following CRISPR-based gene editing in mosquitoes: lessons in how to keep any repair disruptions local.
Strategies to improve homology-based repair outcomes following CRISPR-based gene editing in mosquitoes: lessons in how to keep any repair disruptions local.
复制标题
DOI：
10.1186/s12985-022-01859-2
复制
发表时间：
2022-07-30
期刊：
影响因子：
4.8
通讯作者：
Adelman, Zach N.
中科院分区：
文献类型：
--
作者：
Finney, Micaela;Romanowski, Joseph;Adelman, Zach N.
CRISPR-Cas等可编程基因编辑系统使蚊子基因组工程变得更加实用和容易，促进了病媒控制的尖端遗传方法的发展。然而，这一进展受到了基于同源定向修复（HDR）的DNA双链断裂（DSB）序列整合效率低下以及对蚊子DSB修复缺乏了解的限制。已经在哺乳动物系统中进行了通过抑制非同源末端连接或促进HDR来优化HDR序列整合的创新努力，然而这些方法中的许多尚未应用于蚊子。在这里，我们回顾了DNA DSB修复选择的一些最相关的步骤，并强调了在蚊子基因编辑的背景下影响这种选择以增强HDR的有前途的方法。
Programmable gene editing systems such as CRISPR-Cas have made mosquito genome engineering more practical and accessible, catalyzing the development of cutting-edge genetic methods of disease vector control. This progress, however, has been limited by the low efficiency of homology-directed repair (HDR)-based sequence integration at DNA double-strand breaks (DSBs) and a lack of understanding about DSB repair in mosquitoes. Innovative efforts to optimize HDR sequence integration by inhibiting non-homologous end joining or promoting HDR have been performed in mammalian systems, however many of these approaches have not been applied to mosquitoes. Here, we review some of the most relevant steps of DNA DSB repair choice and highlight promising approaches that influence this choice to enhance HDR in the context of mosquito gene editing.
登录
查看更多内容
DOI：
10.1126/science.aaa5945
发表时间：
2015-04-24
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Gantz VM;Bier E
通讯作者：
Bier E
影响因子：
16.6
作者：
Carlson-Stevermer J;Abdeen AA;Kohlenberg L;Goedland M;Molugu K;Lou M;Saha K
通讯作者：
Saha K
影响因子：
3.8
作者：
Carvalho DO;McKemey AR;Garziera L;Lacroix R;Donnelly CA;Alphey L;Malavasi A;Capurro ML
通讯作者：
Capurro ML
DOI：
10.1073/pnas.121046198
发表时间：
2001-07-17
影响因子：
11.1
作者：
Gasior, SL;Olivares, H;Bishop, DK
通讯作者：
Bishop, DK
影响因子：
16.8
作者：
通讯作者：
--