Direct Enantiomeric Separation and Determination of Hexythiazox Enantiomers in Environment and Vegetable by Reverse-Phase High-Performance Liquid Chromatography
Direct Enantiomeric Separation and Determination of Hexythiazox Enantiomers in Environment and Vegetable by Reverse-Phase High-Performance Liquid Chromatography
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反相高效液相色谱法直接对映体分离和测定环境和蔬菜中的噻唑酮对映体
DOI：
10.3390/ijerph17103453
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发表时间：
2020-05
影响因子：
--
通讯作者：
He Lin
中科院分区：
文献类型：
--
作者：
Zhang Ping;Wang Sheng;Shi Dongmei;Xu Yangyang;Yang Furong;Deng Xile;He Yuhan;He Lin
本研究采用反相高效液相色谱法（RP-HPLC），考察了噻螨酮对映体在Lux cellulose-1、Lux cellulose-2、Lux cellulose-3、Lux cellulose-4、Lux amylose-1和Chirapak IC手性柱上的直接拆分。乙腈/水和甲醇/水用作移动的相，流速为0.8 mL·min-1。考察了手性固定相、温度、热力学参数、移动的相组成和移动的相比对噻螨酮对映体拆分的影响。Hexythiazox对映体在Lux cellulose-3柱上得到基线分离，最大分离度分别为Rs = 2.09（甲醇/水）和Rs = 2.74（乙腈/水）。在其他5个手性柱上均实现了部分分离。当以甲醇/水为移动的流动相时，Lux Amylose-1和Chirapak IC对噻螨酮对映体没有分离能力。温度研究表明，柱温从10 °C升高到40 °C，容量因子（k）和分离因子（Rs）均降低。计算了噻螨酮分离过程中的焓（ΔH）和熵（ΔS），结果表明，较低的温度有利于噻螨酮的分离。此外，还建立了反相高效液相色谱条件下噻螨酮对映体在环境（土壤和水）和蔬菜（黄瓜、白菜和番茄）中的残留分析方法，该方法准确度和精密度可靠。该结果为噻螨酮对映体在反相条件下的基线分离提供了方法，有助于噻螨酮对映体水平的环境和健康风险评估。
In the present study, the direct enantiomeric separation of hexythiazox enantiomers on Lux cellulose-1, Lux cellulose-2, Lux cellulose-3, Lux cellulose-4, Lux amylose-1 and Chirapak IC chiral columns were carefully investigated by reverse-phase high-performance liquid chromatography (RP-HPLC). Acetonitrile/water and methanol/water were used as mobile phase at a flow rate of 0.8 mL·min−1. The effects of chiral stationary phase, temperature, thermodynamic parameters, mobile phase component and mobile phase ratio on hexythiazox enantiomers separation were fully evaluated. Hexythiazox enantiomers received a baseline separation on the Lux cellulose-3 column with a maximum resolution of Rs = 2.09 (methanol/water) and Rs = 2.74 (acetonitrile/water), respectively. Partial separations were achieved on other five chiral columns. Furthermore, Lux amylose-1 and Chirapak IC had no separation ability for hexythiazox enantiomers when methanol/water was used as mobile phase. Temperature study indicated that the capacity factor (k) and resolution factor (Rs) decreased with column temperature increasing from 10 °C to 40 °C. The enthalpy (ΔH) and entropy (ΔS) involved in hexythiazox separation were also calculated and demonstrated the lower temperature contributed to better separation resolution. Moreover, the residue analytical method for hexythiazox enantiomers in the environment (soil and water) and vegetable (cucumber, cabbage and tomato) were also established with reliable accuracy and precision under reverse-phase HPLC condition. Such results provided a baseline separation method for hexythiazox enantiomers under reverse-phase conditions and contributed to an environmental and health risk assessment of hexythiazox at enantiomer level.
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高效液相色谱对映选择性分离分析手性农药
影响因子：
13.1
作者：
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通讯作者：
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影响因子：
1.6
作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
Himdata Abdourahime;M. Anastassiadou;Alba Brancato;D. Brocca;Luis Carrasco Cabrera;Chloé De Lentdecker;Lucien Ferreira;Luna Greco;Samira Jarrah;Dimitra Kardassi;R. Leuschner;A. Lostia;C. Lythgo;P. Medina;Ileana Miron;Tunde Molnar;Stéfanie Nave;R. Pedersen;Marianna Raczyk;H. Reich;Silvia Ruocco;Angela Sacchi;Miguel Santos;A. Stanek;Juergen Sturma;J. Tarazona;A. Theobald;B. Vagenende;A. Verani;Laura Villamar‐Bouza
影响因子：
2
作者：
Wang, Peng;Liu, Donghui;Zhou, Zhiqiang
通讯作者：
Zhou, Zhiqiang
影响因子：
4.7
作者：
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通讯作者：
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