Identification of (-)-epigallocatechin-3-gallate as a potential agent for blocking infection by grass carp reovirus
Identification of (-)-epigallocatechin-3-gallate as a potential agent for blocking infection by grass carp reovirus
复制标题
鉴定 (-)-表没食子儿茶素-3-没食子酸酯作为阻断草鱼呼肠孤病毒感染的潜在药物
DOI：
10.1007/s00705-016-2751-9
复制
发表时间：
2016-01
影响因子：
2.7
通讯作者：
Lu Liqun
中科院分区：
文献类型：
--
作者：
Wang Hao;Liu Weisha;Yu Fei;Lu Liqun
草鱼呼肠孤病毒（GCRV）是水生病毒C种的代表株，是研究水生病毒发病机制的模型。此前，表没食子儿茶素没食子酸酯（EGCG）已被证明可以抑制正呼肠孤病毒感染。本研究的目的是测试其阻止 GCRV 感染的潜力。我们发现，GCRV 颗粒对 CIK（Ctenopharyngodon idelluskidney）细胞表面的粘附受到 EGCG 以及绿茶粗提取物的剂量依赖性抑制。我们还使用 CIK 细胞评估了 EGCG 和绿茶提取物的安全性，结果表明 EGCG 是一种有前途的化合物，可开发为植物源小分子治疗剂，治疗 GCRV 感染引起的草鱼出血性疾病。作为 37/67-kDa 层粘连蛋白受体 (LamR) 的配体，EGCG 对 GCRV 附着的阻断作用与 GCRV 颗粒与 LamR 的结合潜力相关，这是从 VOPBA 测定中推断出来的。
Grass carp reovirus (GCRV), the representative strain of the speciesAquareovirus C, serves as a model for studying the pathogenesis of aquareoviruses. Previously, epigallocatechin gallate (EGCG) was shown to inhibit orthoreovirus infection. The aim of this study was to test its potential in blocking infection by GCRV. We show that adhesion to the CIK (Ctenopharyngodon idelluskidney) cell surface by GCRV particles is inhibited in a dose-dependent manner by EGCG, as well as by a crude extract of green tea. We also evaluated the safety of EGCG and green tea extract using CIK cells, and the results suggest that EGCG is a promising compound that may be developed as a plant-derived small molecular therapeutic agent against grass carp hemorrhagic disease caused by GCRV infection. As the ligand for the 37/67-kDa laminin receptor (LamR), EGCG’s blocking effect on GCRV attachment was associated with the binding potential of GCRV particles to LamR, which was inferred from a VOPBA assay.
登录
查看更多内容
影响因子：
5.4
作者：
Akache, Bassel;Grimm, Dirk;Kay, Mark A.
通讯作者：
Kay, Mark A.
DOI：
10.1201/b15323-24
发表时间：
2013-08
期刊：
--
影响因子：
--
作者：
N. Khan;H. Mukhtar
通讯作者：
N. Khan;H. Mukhtar
影响因子：
6.2
作者：
M. Price;C. Tennant;Ross C. Smith;P. Butow;Susan J. Kennedy;M. Kossoff;S. Dunn
通讯作者：
M. Price;C. Tennant;Ross C. Smith;P. Butow;Susan J. Kennedy;M. Kossoff;S. Dunn
DOI：
10.1016/0167-5699(94)90158-9
发表时间：
1994-03
期刊：
Immunology today
影响因子：
--
作者：
W. Ahne
通讯作者：
W. Ahne
影响因子：
4.1
作者：
Rao Y;Su J
通讯作者：
Su J