Dual patch voltage clamp study of low membrane resistance astrocytes in situ.
Dual patch voltage clamp study of low membrane resistance astrocytes in situ.
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DOI：
10.1186/1756-6606-7-18
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发表时间：
2014-03-17
期刊：
影响因子：
3.6
通讯作者：
Zhou M
中科院分区：
文献类型：
--
作者：
Ma B;Xu G;Wang W;Enyeart JJ;Zhou M
全细胞膜片钳记录已成功地用于鉴定各种细胞中离子通道的电压依赖性门控和电导特性。然而，这种强大的技术是有限的价值，在研究低膜电阻细胞，如星形胶质细胞在原位，因为无法控制或准确地测量命令电压的真实的幅度。为了促进星形胶质细胞离子电导的研究，我们开发了一种双贴片记录方法，该方法允许同时记录星形胶质细胞的膜电流和膜电位，尽管它们的膜电阻非常低。通过测量星形胶质细胞中大量表达的内向整流K+电流的电压依赖性激活和与星形胶质细胞GABAA受体激活相关的多个离子事件来证明该技术的实用性。该方案可以在星形胶质细胞的研究中常规进行。这种方法将是有价值的识别和表征的个别离子通道，编排的电活动的低膜电阻细胞。
Whole-cell patch clamp recording has been successfully used in identifying the voltage-dependent gating and conductance properties of ion channels in a variety of cells. However, this powerful technique is of limited value in studying low membrane resistance cells, such as astrocytes in situ, because of the inability to control or accurately measure the real amplitude of command voltages. To facilitate the study of ionic conductances of astrocytes, we have developed a dual patch recording method which permits membrane current and membrane potential to be simultaneously recorded from astrocytes in spite of their extraordinarily low membrane resistance. The utility of this technique is demonstrated by measuring the voltage-dependent activation of the inwardly rectifying K+ current abundantly expressed in astrocytes and multiple ionic events associated with astrocytic GABAA receptor activation. This protocol can be performed routinely in the study of astrocytes. This method will be valuable for identifying and characterizing the individual ion channels that orchestrate the electrical activity of low membrane resistance cells.
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影响因子：
5.3
作者：
Djukic, Biljana;Casper, Kristen B.;McCarthy, Ken D.
通讯作者：
McCarthy, Ken D.
影响因子：
3.6
作者：
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通讯作者：
Lee CJ
影响因子：
5.3
作者：
Wang W;Putra A;Schools GP;Ma B;Chen H;Kaczmarek LK;Barhanin J;Lesage F;Zhou M
通讯作者：
Zhou M
影响因子：
3.3
作者：
Kang, J.;Kang, N.;Yu, Y.;Zhang, J.;Petersen, N.;Tian, G. F.;Nedergaard, M.
通讯作者：
Nedergaard, M.
影响因子：
3.4
作者：
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通讯作者：
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