Characteristics of gene expression in frozen shoulder.
Characteristics of gene expression in frozen shoulder.
复制标题
DOI：
10.1186/s12891-022-05762-3
复制
发表时间：
2022-08-25
影响因子：
2.3
通讯作者：
中科院分区：
文献类型：
--
作者：
严重的冻结肩（FS）通常对治疗有抵抗力，因此可能导致长期的功能障碍。然而，其病因仍然不明。我们假设FS的基因表达会因滑膜部位而异。前瞻性收集FS患者的滑膜组织，分析19个基因的表达。还分析了肩袖撕裂（RCT）或肩关节不稳定（SI）患者的滑膜组织作为对照。每组共分析10份样本。关节镜下从三个不同位置采集标本：肩袖间隙（RI）、腋隐窝（AX）和肩峰下囊（SAB）。从收集的组织中提取总RNA，并通过实时聚合酶链反应分析以下基因：基质金属蛋白酶（MMP）;金属蛋白酶组织抑制剂（TIMP）;炎性细胞因子（IL 1B、TNF和IL 6）; I型和II型前胶原（COL 1A 1和COL 2A 1）;生长因子（IGF 1和TGFB 1）;神经因子（NGF和NGFR）; SOX 9和ACTA 2。位点特异性分析显示，MMP 13、IL-6、SOX 9和COL 1A 1在所有三个位点均升高。FS组AX中MMP 3、MMP 9、COL 2A 1和NGFR四种基因表达均高于RCT组和SI组，RI中MMP 3和SAB中NGFR表达均高于RCT组和SI组。在FS组中，与软骨形成（MMP 2、IGF 1、SOX 9、COL 2A 1、NGF和NGFR）或纤维化（MMP 9、TGFB 1和COL 1A 1）相关的基因表达之间存在相关性。FS组中许多MMPs、促炎细胞因子和胶原相关基因的表达水平增加，表明分解代谢和合成代谢变化同时发生。此外，与软骨形成或纤维化相关的基因在FS组中高度表达，这可能影响了肩关节的活动范围限制。与RI和SAB相比，AX是FS中表达增加的最常见部位。分析肩关节下部可能有助于阐明FS的发病机制。
Severe frozen shoulder (FS) is often resistant to treatment and can thus result in long-term functional impairment. However, its etiology remains unknown. We hypothesized that gene expression of FS would vary by synovial location. The synovial tissues of patients with FS were collected prospectively and analyzed for the expression of 19 genes. Synovial tissues from patients with rotator cuff tear (RCT) or shoulder instability (SI) were also analyzed as controls. A total of 10 samples were analyzed from each group. The specimens were arthroscopically taken from three different locations: rotator interval (RI), axillary recess (AX), and subacromial bursa (SAB). Total RNA was extracted from the collected tissues and was analyzed by real-time polymerase chain reaction for the following genes: matrix metalloproteinases (MMPs); tissue inhibitors of metalloproteinases (TIMPs); inflammatory cytokines (IL1B, TNF, and IL6); type I and II procollagen (COL1A1 and COL2A1); growth factors (IGF1 and TGFB1); neural factors (NGF and NGFR); SOX9; and ACTA2. Site-specific analysis showed that MMP13, IL-6, SOX9, and COL1A1 were increased in all three sites. Four genes (MMP3, MMP9, COL2A1, and NGFR) were increased in the AX, MMP3 in the RI, and NGFR in the SAB were increased in the FS group than in the RCT and SI groups. In the FS group, there was a correlation between the expression of genes related to chondrogenesis (MMP2, IGF1, SOX9, COL2A1, NGF, and NGFR) or fibrosis (MMP9, TGFB1, and COL1A1). The expression levels of numerous MMPs, pro-inflammatory cytokines, and collagen-related genes were increased in the FS group, suggesting that catabolic and anabolic changes have simultaneously occurred. In addition, genes related to chondrogenesis or fibrosis were highly expressed in the FS group, which might have affected the range of motion limitation of the shoulder. Compared to RI and SAB, the AX was the most common site of increased expression in FS. Analyzing the lower region of the shoulder joint may lead to the elucidation of the pathogenesis of FS.
登录
查看更多内容
影响因子：
9
作者：
Lu Z;Lei D;Jiang T;Yang L;Zheng L;Zhao J
通讯作者：
Zhao J
影响因子：
--
作者：
van der Kraan PM
通讯作者：
van der Kraan PM
影响因子：
--
作者：
Cho CH;Song KS;Kim BS;Kim DH;Lho YM
通讯作者：
Lho YM
影响因子：
2.8
作者：
Rodeo, SA;Hannafin, JA;Wickiewicz, TL
通讯作者：
Wickiewicz, TL
影响因子：
5
作者：
Song, Kyoung Doo;Kwon, Jong Won;Choi, Sang-Hee
通讯作者：
Choi, Sang-Hee