Measurement of endogenous Na+,K+‐ATPase inhibitors in human plasma and urine using high‐performance liquid chromatography
Measurement of endogenous Na+,K+‐ATPase inhibitors in human plasma and urine using high‐performance liquid chromatography
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使用高效液相色谱法测定人血浆和尿液中的内源性 Na+,K+-ATP 酶抑制剂
DOI：
10.1016/0014-5793(84)80946-1
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发表时间：
1984
期刊：
影响因子：
3.5
通讯作者：
J. Cloix
中科院分区：
文献类型：
--
作者：
M. Crabos;Irving W. Wainer;J. Cloix
本研究旨在评估同一受试者血浆和尿液中的内源性Na+，K+-ATP酶抑制剂。使用乙腈梯度在反相HPLC上对样品进行色谱分析，并使用Na+，K+-ATP酶抑制和与抗地高辛抗体的交叉反应筛选洗脱液。使用先前描述的方法，将供体分为抑制性和非抑制性受试者，血浆对哇巴因结合和Na+，K+-ATP酶活性的作用。血浆中可检测到3种Na+，K+-ATP酶抑制剂（IP、2 P和3 P），2 P和3 P峰的抗体交叉反应大于IP峰。与非抑制受试者相比，抑制受试者的峰2 P和3 P显著更高。24 h尿中Na+，K+-ATP酶活性抑制峰分别为1U和2U。峰2U与抗地高辛抗体的交叉反应程度大于峰1U，并且在抑制受试者的Na+，K+-ATP酶抑制方面显著更大。这些数据支持血浆和尿液中人Na+，K+-ATP酶抑制剂的异质性。
This study was undertaken to assess endogenous Na+,K+-ATPase inhibitors in both plasma and urine in the same subjects. Samples were chromatographed on reverse-phase HPLC using an acetonitrile gradient and the eluent screened using Na+,K+-ATPase inhibition and cross-reaction with anti-digoxin antibodies. The donors were divided into inhibiting and non-inhibiting subjects using a previously described method, plasma action on ouabain binding and on Na+,K+-ATPase activity. Three Na+,K+-ATPase inhibitors (IP, 2P and 3P) were detectable in plasma; the antibodies cross-reaction of the peaks 2P and 3P were larger than that of peak IP. The peaks 2P and 3P were significantly higher in inhibiting subjects as compared to non-inhibiting subjects. The 24-h urine is resolved into two peaks inhibiting Na+,K+-ATPase activity (1U and 2U). Peak 2U cross-reacted with anti-digoxin antibodies to a greater extent than peak 1U and is significantly larger in inhibiting subjects in terms of Na+,K+-ATPase inhibition. These data support the heterogeneity of human Na+,K+-ATPase inhibitor in both plasma and urine.
从狗尿中分离纯化钠转运抑制剂的研究进展。
影响因子：
19.6
作者：
Licht,A;Stein,S;McGregor,CW;Bourgoignie,JJ;Bricker,NS
通讯作者：
Bricker,NS