Progress in isolation and purification of an inhibitor of sodium transport obtained from dog urine.
Progress in isolation and purification of an inhibitor of sodium transport obtained from dog urine.
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从狗尿中分离纯化钠转运抑制剂的研究进展。
DOI：
10.1038/ki.1982.27
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发表时间：
1982
影响因子：
19.6
通讯作者：
Bricker,NS
中科院分区：
文献类型：
--
作者：
Licht,A;Stein,S;McGregor,CW;Bourgoignie,JJ;Bricker,NS
方法 我们在研究中遵循的事件顺序列于表 1。 收获对照和盐皮质激素“逃逸”尿液 使用了四只健康成年训练过的雌性杂种狗的群体。每只动物都饲养在单独的代谢笼中。在前 3 天，每天给狗喂食含有 50 mEq 钠的饮食。从第四天开始一直到第十天，钠摄入量增加到每天 250 mEq； 0.1 mg 氟氢可的松，每日两次。在整个 10 天的周期中，从每只狗身上连续采集 24 小时尿液样本。将第一天到第三天所有四只狗的收集物合并起来，用作对照样本。在第五天时，总是会发生从盐皮质激素和高盐养生法中“逃脱”的情况。因此，将第 6 天至第 10 天的 24 小时收集数据合并起来并用作利尿钠因子的来源。第十天结束时，将狗恢复到正常栖息地并喂食正常的实验室食物，不添加氟氢可的松盐。在下一个周期开始之前，他们被允许休息 4 天。采用六个完整的循环来获得研究所需的 150 升尿液。
Methods The sequence of events that we followed in the studies is listed in Table 1.Harvesting control and mineralocorticoid hormone" escape" urine A colony of four healthy adult trained female mongrel dogs was used. Each animal was maintained in an individual metabolic cage. During the first 3 days, the dogs were fed a diet containing 50 mEq of sodium daily. Beginning on the fourth day and continuing through the tenth day, the sodium intake was increased to 250 mEq per day; 0.1 mg of fludrocortisone was administered twice daily. Consecutive 24-hour urine sample collections were obtained from each dog through the full 10-day cycle. The collections from all four dogs were pooled for days I through 3 and were used as the control sample." Escape" from the minerolocorticoid hormone and high salt regimen invariably occurred by the fifth day. Thus, the 24-hour collections from days 6 through 10 were pooled and utilized as the source of the natriuretic factor. At the end of the tenth day, the dogs were restored to their regular habitat and fed normal laboratory chow without added salt of fludrocortisone. They were allowed a rest period of 4 days before the next cycle was initiated. Six complete cycles were employed to obtain the desired 150 liters of urine for study.
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影响因子：
15.9
作者：
BOURGOIGNIE, JJ;HWANG, KH;BRICKER, NS
通讯作者：
BRICKER, NS
从大鼠肾脏提取的利尿钠因子的基础活性作为饮食的函数及其在调节急性钠平衡中的作用。
影响因子：
6
作者：
F. Louis;H. Favre
通讯作者：
H. Favre
肾功能正常的狗尿液中钠转运的抑制剂。
DOI：
10.1172/jci108206
发表时间：
1975
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
H. Favre;K. H. Hwang;R. Schmidt;N. Bricker;J. Bourgoignie
通讯作者：
J. Bourgoignie
来自牛下丘脑的钠转运抑制剂。
DOI：
10.1073/pnas.76.9.4658
发表时间：
1979
影响因子：
11.1
作者：
G. T. Haupert;J. Sancho
通讯作者：
J. Sancho
使用荧光胺自动监测制备柱流出物中的伯胺。
影响因子：
2.9
作者：
Peter Böhlen;S. Stein;J. Stone;S. Udenfriend
通讯作者：
S. Udenfriend