Guidelines for measuring reactive oxygen species and oxidative damage in cells and in vivo.
Guidelines for measuring reactive oxygen species and oxidative damage in cells and in vivo.
复制标题
细胞及体内活性氧物质与氧化损伤的测量指南。
DOI：
10.1038/s42255-022-00591-z
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发表时间：
2022-06
影响因子：
20.8
通讯作者：
Halliwell, Barry
中科院分区：
文献类型：
--
作者：
Murphy, Michael P.;Bayir, Hulya;Belousov, Vsevolod;Chang, Christopher J.;Davies, Kelvin J. A.;Davies, Michael J.;Dick, Tobias P.;Finkel, Toren;Forman, Henry J.;Janssen-Heininger, Yvonne;Gems, David;Kagan, Valerian E.;Kalyanaraman, Balaraman;Larsson, Nils-Goran;Milne, Ginger L.;Nystrom, Thomas;Poulsen, Henrik E.;Radi, Rafael;Van Remmen, Holly;Schumacker, Paul T.;Thornalley, Paul J.;Toyokuni, Shinya;Winterbourn, Christine C.;Yin, Huiyong;Halliwell, Barry
在整个生物科学中，活性氧物种(ROS)的多重作用及其对健康和疾病的影响正在显现。这一发展导致不熟悉ROS及其反应的复杂性的研究人员使用商业试剂盒和探针来不适当地测量ROS和氧化损伤，将ROS(通用缩写)视为一个离散的分子实体。不幸的是，这些测量的应用和解释充满了挑战和限制。这可能会导致误导性的说法进入文献并阻碍进展，尽管关于如何最好地评估单个RO、它们的反应、作为信号分子的作用以及它们可能造成的氧化损伤的知识体系已经确立。在这份共识声明中，我们阐明了许多常用的ROS和氧化损伤测量方法可能出现的问题，并提出了最佳实践的指导方针。我们希望这些策略将对那些发现他们的研究需要评估细胞内和体内的ROS、氧化损伤和氧化还原信号的人有用。
Multiple roles of reactive oxygen species (ROS) and their consequences for health and disease are emerging throughout biological sciences. This development has led researchers unfamiliar with the complexities of ROS and their reactions to employ commercial kits and probes to measure ROS and oxidative damage inappropriately, treating ROS (a generic abbreviation) as if it were a discrete molecular entity. Unfortunately, the application and interpretation of these measurements are fraught with challenges and limitations. This can lead to misleading claims entering the literature and impeding progress, despite a well-established body of knowledge on how best to assess individual ROS, their reactions, role as signalling molecules and the oxidative damage that they can cause. In this consensus statement we illuminate problems that can arise with many commonly used approaches for measurement of ROS and oxidative damage, and propose guidelines for best practice. We hope that these strategies will be useful to those who find their research requiring assessment of ROS, oxidative damage and redox signalling in cells and in vivo.
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影响因子：
82.9
作者：
Breckwoldt, Michael;Pfister, Franz M. J.;Misgeld, Thomas
通讯作者：
Misgeld, Thomas
影响因子：
4.8
作者：
Gedik, CM;Collins, A;Vaughan, N
通讯作者：
Vaughan, N
影响因子：
4.7
作者：
Butterfield DA;Boyd-Kimball D
通讯作者：
Boyd-Kimball D
DOI：
10.1038/s41573-021-00233-1
发表时间：
2021-09
期刊：
Nature reviews. Drug discovery
影响因子：
--
作者：
Forman HJ;Zhang H
通讯作者：
Zhang H
影响因子：
5.5
作者：
Halliwell, Barry
通讯作者：
Halliwell, Barry