OsAP65, a rice aspartic protease, is essential for male fertility and plays a role in pollen germination and pollen tube growth.
OsAP65, a rice aspartic protease, is essential for male fertility and plays a role in pollen germination and pollen tube growth.
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OsAP65 是一种水稻天冬氨酸蛋白酶，对于雄性生育力至关重要，在花粉萌发和花粉管生长中发挥作用
DOI：
10.1093/jxb/ert173
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发表时间：
2013-08
影响因子：
6.9
通讯作者：
Zhang Q
中科院分区：
文献类型：
--
作者：
Huang J;Zhao X;Cheng K;Jiang Y;Ouyang Y;Xu C;Li X;Xiao J;Zhang Q
天冬氨酸蛋白酶（AP）是一个广泛存在于动物、微生物、病毒和植物中的蛋白水解酶家族。水稻基因组编码96个AP，其中只有少数已被功能化。在这里，一个新的AP基因，OsAP 65，它在水稻花粉管生长中起着不可或缺的作用，的鉴定和表征。OsAP 65的T-DNA插入系造成严重的分离畸变。在来自T-DNA插入杂合子个体的后代中，野生型和携带T-DNA的杂合子以接近1：1的比例分离，而破坏的OsAP 65（OsAP 65-/-）的纯合子没有回收。杂合子和野生型植物之间的相互杂交表明，突变等位基因不能通过雄配子传递。对杂合植株花药的检查显示，突变体花粉正常成熟，但不萌发或伸长。OsAP 65在各种组织中表达，并且在杂合植物中的转录水平是在野生型植物中测量的量的约一半。亚细胞定位结果表明OsAP 65是一个前液泡区室（PVC）蛋白。这些结果表明，OsAP 65是水稻花粉萌发和花粉管生长所必需的。
Aspartic proteases (APs) comprise a large proteolytic enzyme family widely distributed in animals, microbes, viruses, and plants. The rice genome encodes 96 APs, of which only a few have been functionally characterized. Here, the identification and characterization of a novel AP gene, OsAP65, which plays an indispensable role in pollen tube growth in rice, is reported. The T-DNA insertion line of OsAP65 caused severe segregation distortion. In the progeny derived from an individual heterozygous for the T-DNA insertion, the wild type and T-DNA-carrying heterozygote segregated at a ratio close to 1:1, while homozygotes of disrupted OsAP65 (OsAP65–/–) were not recovered. Reciprocal crosses between heterozygotes and wild-type plants demonstrated that the mutant alleles could not be transmitted through the male gamete. Examination of the anthers from heterozygous plants revealed that the mutant pollen matured normally, but did not germinate or elongate. OsAP65 was expressed in various tissues and the transcript level in heterozygous plants was about half of the amount measured in the wild-type plants. The subcellular localization showed that OsAP65 is a pre-vacuolar compartment (PVC) protein. These results indicated that OsAP65 was essential for rice pollen germination and tube growth.
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影响因子：
7.2
作者：
Jeong, DH;An, S;An, G
通讯作者：
An, G
影响因子：
11.6
作者：
Jin, JB;Bae, HJ;Hwang, IW
通讯作者：
Hwang, IW
DOI：
10.1073/pnas.0804761105
发表时间：
2008-08-12
影响因子：
11.1
作者：
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通讯作者：
Zhang, Qifa
DOI：
10.1073/pnas.1734110100
发表时间：
2003-09-02
影响因子：
11.1
作者：
Golovkin, M;Reddy, ASN
通讯作者：
Reddy, ASN
影响因子：
3.8
作者：
Han, Min-Jung;Jung, Ki-Hong;An, Gynheung
通讯作者：
An, Gynheung