The correlation of methylation levels measured using Illumina 450K and EPIC BeadChips in blood samples.
The correlation of methylation levels measured using Illumina 450K and EPIC BeadChips in blood samples.
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DOI：
10.2217/epi-2017-0078
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发表时间：
2017-11
期刊：
影响因子：
3.8
通讯作者：
Miller MW
中科院分区：
文献类型：
--
作者：
Logue MW;Smith AK;Wolf EJ;Maniates H;Stone A;Schichman SA;McGlinchey RE;Milberg W;Miller MW
我们检查了Illumina Infinium HumanMethylation450 BeadChip和Infinium MethylationEPIC BeadChip之间甲基化水平的一致性。我们计算了145个全血DNA样本在422，524个CpG位点中的每一个位点上的相关性。在某些地点的相关性很高（r = 0.95），但许多地点的相关性很低（55%的r < 0.20）。在许多基因座处450K和EPIC测量的甲基化值之间的低对应性主要是由于血液中大多数CpG位点的甲基化值的低变异性。根据观察到的相关性或低变异性过滤掉探针可能会增加基于BeadChip的流行病学研究的重现性。
We examined concordance of methylation levels across the Illumina Infinium HumanMethylation450 BeadChip and the Infinium MethylationEPIC BeadChip. We computed the correlation for 145 whole blood DNA samples at each of the 422,524 CpG sites measured by both chips. The correlation at some sites was high (up to r = 0.95), but many sites had low correlation (55% had r < 0.20). The low correspondence between 450K and EPIC measured methylation values at many loci was largely due to the low variability in methylation values for the majority of the CpG sites in blood. Filtering out probes based on the observed correlation or low variability may increase reproducibility of BeadChip-based epidemiological studies.
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影响因子：
11
作者：
Sadeh N;Spielberg JM;Logue MW;Wolf EJ;Smith AK;Lusk J;Hayes JP;Sperbeck E;Milberg WP;McGlinchey RE;Salat DH;Carter WC;Stone A;Schichman SA;Humphries DE;Miller MW
通讯作者：
Miller MW
影响因子：
3.8
作者：
Lee MK;Hong Y;Kim SY;Kim WJ;London SJ
通讯作者：
London SJ
全基因组甲基化谱揭示了人类衰老速度的定量观点。
影响因子：
16
作者：
Hannum, Gregory;Guinney, Justin;Zhao, Ling;Zhang, Li;Hughes, Guy;Sadda, SriniVas;Klotzle, Brandy;Bibikova, Marina;Fan, Jian-Bing;Gao, Yuan;Deconde, Rob;Chen, Menzies;Rajapakse, Indika;Friend, Stephen;Ideker, Trey;Zhang, Kang
通讯作者：
Zhang, Kang
影响因子：
25.8
作者：
Montano C;Taub MA;Jaffe A;Briem E;Feinberg JI;Trygvadottir R;Idrizi A;Runarsson A;Berndsen B;Gur RC;Moore TM;Perry RT;Fugman D;Sabunciyan S;Yolken RH;Hyde TM;Kleinman JE;Sobell JL;Pato CN;Pato MT;Go RC;Nimgaonkar V;Weinberger DR;Braff D;Gur RE;Fallin MD;Feinberg AP
通讯作者：
Feinberg AP
影响因子：
4.9
作者：
Butler, Andrew C.;Godbole, Namrata;Marsh, Elizabeth J.
通讯作者：
Marsh, Elizabeth J.