Hierarchical nanoengineered surfaces for enhanced cytoadhesion and drug delivery.
Hierarchical nanoengineered surfaces for enhanced cytoadhesion and drug delivery.
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DOI：
10.1016/j.biomaterials.2011.01.022
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发表时间：
2011-05
期刊：
影响因子：
14
通讯作者：
Desai, Tejal A.
中科院分区：
文献类型：
--
作者：
Fischer, Kathleen E.;Nagaraj, Ganesh;Daniels, R. Hugh;Li, Esther;Cowles, Verne E.;Miller, Jennifer L.;Bunger, Mark D.;Desai, Tejal A.
将治疗剂递送到粘膜组织如鼻和胃肠道是高度期望的，这是由于易于进入和密集的脉管系统。然而，粘液层有效地捕获和去除大多数治疗性大分子和装置。在以前的工作中，我们已经表明，纳米工程微粒（NEMPs）粘附通过粘液层，表现出高达1000倍的未改性微球的拉脱力，并显示出更大的粘附力比一些化学靶向手段。在本文中，我们证明了纳米形貌改善了器件在体内的粘附性，使保留时间比未改性的器件增加了10倍。此外，我们观察到相当大的粘附在几个细胞系使用体外剪切流模型，表明这种方法是有前途的许多组织。然后，我们证明，纳米线介导的粘附是高度稳健的纳米线表面电荷和细胞结构和功能的变化，我们表征颗粒加载和洗脱。我们提出了一种形式的细胞粘附，利用纳米工程表面与亚细胞结构的物理相互作用，产生一个强大的和多功能的细胞粘附药物输送。这些纳米级粘附机制也与诸如组织工程和伤口愈合的领域相关，因为它们可能影响干细胞分化、细胞重塑、迁移等。
Delivering therapeutics to mucosal tissues such as the nasal and gastrointestinal tracts, is highly desirable due to ease of access and dense vasculature. However, the mucus layer effectively captures and removes most therapeutic macromolecules and devices. In previous work, we have shown that nanoengineered microparticles (NEMPs) adhere through the mucus layer, exhibiting up to 1000 times the pull-off force of an unmodified microsphere, and showing greater adhesion than some chemical targeting means. In this paper, we demonstrate that nanotopography improves device adhesion in vivo, increasing retention time up to ten-fold over unmodified devices. Moreover, we observe considerable adhesion in several cell lines using an in vitro shear flow model, indicating that this approach is promising for numerous tissues. We then demonstrate that nanowire-mediated adhesion is highly robust to variation in nanowire surface charge and cellular structure and function, and we characterize particle loading and elution. We present a form of cytoadhesion that utilizes the physical interaction of nanoengineered surfaces with subcellular structures to produce a robust and versatile cytoadhesive for drug delivery. These nanoscale adhesive mechanisms are also relevant to fields such as tissue engineering and wound healing because they likely affect stem cell differentiation, cell remodeling, migration, etc.
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