Visualizing replication fork encounters with DNA interstrand crosslinks.
Visualizing replication fork encounters with DNA interstrand crosslinks.
复制标题
DOI：
10.1016/bs.mie.2021.08.015
复制
发表时间：
2021
影响因子：
--
通讯作者：
中科院分区：
文献类型：
--
作者：
Replication forks encounter numerous challenges as they move through eu- and hetero-chromatin during S phase in mammalian cells. These include a variety of impediments to the unwinding of DNA by the replicative helicase such as alternate DNA structures, transcription complexes and R-loops, DNA-protein complexes, and DNA chemical adducts. Much of our knowledge of these events is based on analysis of markers of the replication stress and DNA Damage Response that follow stalling of replisomes. To examine consequences for the replisomes more directly we developed an approach for imaging collisions of replication forks with the potent block presented by an interstrand crosslink (ICL). The strategy is based on the visualization on DNA fibers of the encounter of replication tracts and an antigen tagged ICL. Our studies revealed an unexpected restart of DNA synthesis past an intact ICL. In addition, and also unexpected, we found two distinct versions of the replisome, one biased towards euchromatin and the other more prominent in heterochromatin. Here we present details of our experimental procedures that led to these observations.
登录
查看更多内容
影响因子：
3.3
作者：
Bell SP;Labib K
通讯作者：
Labib K
影响因子：
16
作者：
Taylor MRG;Yeeles JTP
通讯作者：
Yeeles JTP
影响因子：
8.8
作者：
Mutreja K;Krietsch J;Hess J;Ursich S;Berti M;Roessler FK;Zellweger R;Patra M;Gasser G;Lopes M
通讯作者：
Lopes M
影响因子：
5.6
作者：
RUPP, WD;HOWARDFL.P
通讯作者：
HOWARDFL.P
影响因子：
21.3
作者：
Gambus, A;Jones, RC;Labib, K
通讯作者：
Labib, K