The reduced kinome of Ostreococcus tauri: core eukaryotic signalling components in a tractable model species.
The reduced kinome of Ostreococcus tauri: core eukaryotic signalling components in a tractable model species.
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DOI：
10.1186/1471-2164-15-640
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发表时间：
2014-08-02
期刊：
影响因子：
4.4
通讯作者：
Millar AJ
中科院分区：
文献类型：
--
作者：
Hindle MM;Martin SF;Noordally ZB;van Ooijen G;Barrios-Llerena ME;Simpson TI;Le Bihan T;Millar AJ
真核生物信号的当前知识来源于表型多样的生物体。有一个迫切的需要，以确定真核生物中保守的信号成分，这将导致跨王国的知识转移。用于信号传导的真核生物模型的两个有用性质是（1）降低的信号传导复杂性，和（2）信号传导组分的保守性。Tauri链球菌被描述为最小的自由生活的真核生物。它的基因不到8,000个，代表了一个高度受限的基因组调色板。我们的研究揭示了133种蛋白激酶和34种蛋白磷酸酶（分别占蛋白质组的1.7%和0.4%）。我们进行了磷酸化蛋白质组学实验，并构建了催化蛋白激酶的结构域结构和同源性。对于每一个主要的激酶家族，我们审查的完整性和分歧的O。tauri代表与实验室模型拟南芥和酿酒酵母以及智人的充分研究的激酶组进行比较。O. tauri减少到一个单一的成员，在家庭多样性的损失，而TKL和ABC 1分支扩大。我们还鉴定了在A. thaliana，但保留在O.金牛座。对于三个，对比真核生物途径- TOR，MAPK和生物钟-我们建立了保守组分的子集，并证明了底物磷酸化和激酶基序的保守位点。我们的结论是O. tauri满足了我们的两个核心要求。它的几种激酶与H. sapiens的直系同源物比S. cerevisiae为H.智人O.因此，Tauri是自由生活的真核生物中信号传导的合适模型。本文的在线版本（doi：10.1186/1471-2164-15-640）包含补充材料，可供授权用户使用。
The current knowledge of eukaryote signalling originates from phenotypically diverse organisms. There is a pressing need to identify conserved signalling components among eukaryotes, which will lead to the transfer of knowledge across kingdoms. Two useful properties of a eukaryote model for signalling are (1) reduced signalling complexity, and (2) conservation of signalling components. The alga Ostreococcus tauri is described as the smallest free-living eukaryote. With less than 8,000 genes, it represents a highly constrained genomic palette. Our survey revealed 133 protein kinases and 34 protein phosphatases (1.7% and 0.4% of the proteome). We conducted phosphoproteomic experiments and constructed domain structures and phylogenies for the catalytic protein-kinases. For each of the major kinases families we review the completeness and divergence of O. tauri representatives in comparison to the well-studied kinomes of the laboratory models Arabidopsis thaliana and Saccharomyces cerevisiae, and of Homo sapiens. Many kinase clades in O. tauri were reduced to a single member, in preference to the loss of family diversity, whereas TKL and ABC1 clades were expanded. We also identified kinases that have been lost in A. thaliana but retained in O. tauri. For three, contrasting eukaryotic pathways – TOR, MAPK, and the circadian clock – we established the subset of conserved components and demonstrate conserved sites of substrate phosphorylation and kinase motifs. We conclude that O. tauri satisfies our two central requirements. Several of its kinases are more closely related to H. sapiens orthologs than S. cerevisiae is to H. sapiens. The greatly reduced kinome of O. tauri is therefore a suitable model for signalling in free-living eukaryotes. The online version of this article (doi:10.1186/1471-2164-15-640) contains supplementary material, which is available to authorized users.
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DOI：
10.1073/pnas.0604511103
发表时间：
2006-07-11
影响因子：
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作者：
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