Sarecycline Demonstrated Reduced Activity Compared to Minocycline against Microbial Species Representing Human Gastrointestinal Microbiota.
Sarecycline Demonstrated Reduced Activity Compared to Minocycline against Microbial Species Representing Human Gastrointestinal Microbiota.
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DOI：
10.3390/antibiotics11030324
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发表时间：
2022-02-28
期刊：
影响因子：
--
通讯作者：
Grada A
中科院分区：
文献类型：
--
作者：
Ghannoum MA;Long L;Bunick CG;Del Rosso JQ;Gamal A;Tyring SK;McCormick TS;Grada A
长期使用广谱四环素类抗生素（如米诺环素和多西环素）可能会显著改变肠道和皮肤微生物组，导致生态失调。Sarecycline是一种用于痤疮治疗的窄谱四环素类抗生素，被假设对胃肠道微生物群的影响最小。我们使用体外最小抑菌浓度（MIC）和时间杀灭动力学测定法评估了与米诺环素相比，盐芥碱对一组微生物的作用，所述微生物反映了肠道微生物组的多样性。与米诺环素相比，盐藻碱显示出较低的抗微生物活性，表现为对拟杆菌门12种分离株中的10种、放线菌门4种分离株中的3种和厚壁菌门7种分离株中的5种的MIC较高，对费氏丙酸杆菌的MIC值显著较高（≥3个稀释度）。在时间-杀灭测定中，在所有时间点，与米诺环素相比，鼠尾草素显示出对大肠杆菌的活性显著更低（p < 0.05）。此外，sarecycline是显着降低有效抑制热带念珠菌相比，米诺环素暴露后20和22小时。此外，盐芥碱显示出对副干酪乳杆菌（最近重新命名为副干酪乳杆菌亚种）的显著更低的活性。paracasei）（p = 0.002）和双歧杆菌（p = 0.042）。总的来说，sarecycline对79%的测试肠道微生物表现出降低的抗微生物活性，这表明与米诺环素相比，它对肠道微生物群的破坏性较小。进一步的体内试验是必要的。
Prolonged use of broad-spectrum tetracycline antibiotics such as minocycline and doxycycline may significantly alter the gut and skin microbiome leading to dysbiosis. Sarecycline, a narrow-spectrum tetracycline-class antibiotic used for acne treatment, is hypothesized to have minimal impact on the gastrointestinal tract microbiota. We evaluated the effect of sarecycline compared to minocycline against a panel of microorganisms that reflect the diversity of the gut microbiome using in vitro minimum inhibitory concentration (MIC) and time-kill kinetic assays. Compared to minocycline, sarecycline showed less antimicrobial activity indicated by higher MIC against 10 of 12 isolates from the Bacteroidetes phylum, three out of four isolates from Actinobacteria phylum, and five of seven isolates from the Firmicutes phylum, with significantly higher MIC values against Propionibacterium freudenreichii (≥3 dilutions). In time-kill assays, sarecycline demonstrated significantly less activity against Escherichia coli compared to minocycline at all time-points (p < 0.05). Moreover, sarecycline was significantly less effective in inhibiting Candida tropicalis compared to minocycline following 20- and 22-h exposure. Furthermore, sarecycline showed significantly less activity against Lactobacillus paracasei (recently renamed as Lacticaseibacillus paracasei subsp. paracasei) (p = 0.002) and Bifidobacterium adolescentis at 48 h (p = 0.042), when compared to minocycline. Overall, sarecycline demonstrated reduced antimicrobial activity against 79% of the tested gut microorganisms, suggesting that it is less disruptive to gut microbiota compared with minocycline. Further in vivo testing is warranted.
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影响因子：
15.9
作者：
Huffnagle, Gary B.;Noverr, Mairi C.
通讯作者：
Noverr, Mairi C.
影响因子：
30.3
作者：
Kelly CJ;Zheng L;Campbell EL;Saeedi B;Scholz CC;Bayless AJ;Wilson KE;Glover LE;Kominsky DJ;Magnuson A;Weir TL;Ehrentraut SF;Pickel C;Kuhn KA;Lanis JM;Nguyen V;Taylor CT;Colgan SP
通讯作者：
Colgan SP
影响因子：
29
作者：
Donohoe DR;Garge N;Zhang X;Sun W;O'Connell TM;Bunger MK;Bultman SJ
通讯作者：
Bultman SJ
DOI：
10.1016/j.juro.2015.08.070
发表时间：
2016-02
期刊：
The Journal of urology
影响因子：
--
作者：
Barnett C;Nazzal L;Goldfarb DS;Blaser MJ
通讯作者：
Blaser MJ
影响因子：
4.9
作者：
Ben-Ami, Ronen;Olshtain-Pops, Keren;Giladi, Michael
通讯作者：
Giladi, Michael