Assessing the Resident Progenitor Cell Population and the Vascularity of the Adult Human Meniscus.
Assessing the Resident Progenitor Cell Population and the Vascularity of the Adult Human Meniscus.
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DOI：
10.1016/j.arthro.2020.09.021
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发表时间：
2021-01
期刊：
影响因子：
--
通讯作者：
Sheyn D
中科院分区：
文献类型：
--
作者：
Chahla J;Papalamprou A;Chan V;Arabi Y;Salehi K;Nelson TJ;Limpisvasti O;Mandelbaum BR;Tawackoli W;Metzger MF;Sheyn D
鉴定、表征和比较新鲜采集的人尸体直肠的红-红、红-白色和白色-白色（WW）区域内的驻留祖细胞群，并使用免疫荧光和三维（3D）成像表征人直肠的血管分布。从健康供体收获新鲜的成年人睾丸。酶消化半月板，分离单核细胞，并使用针对间充质干细胞表面标志物（CD 105、CD 90、CD 44和CD 29）的抗体进行流式细胞术表征。细胞在培养中扩增，表征，并与骨髓来源的间充质干细胞进行比较。测定培养细胞的三系分化潜能。使用改良的uDisco清除和针对血管标记物CD 31、凝集素和α平滑肌肌动蛋白的免疫荧光，在3D中绘制了血管的血管结构。3个区域之间分离细胞的克隆形成性无显著差异。流式细胞术显示，所有3个区均存在CD 44 + CD 105 + CD 29 + CD 90+细胞，WW区的患病率较高。来自所有区域的祖细胞被发现是有效的分化为间充质谱系。观察到半月板红-红区中的较大血管向红-白白色延伸，出芽为较小的小动脉和小静脉。使用3D成像在所有区域中鉴定CD 31+细胞，并观察到血管系统的其他标志物（凝集素和α平滑肌肌动蛋白）的共定位。无损伤的健康成人供体的所有3个小脑区中均明显存在常驻间充质祖细胞。此外，我们的研究结果表明，在WW区的血管化的存在。
To identify, characterize, and compare the resident progenitor cell populations within the red‒red, red‒white, and white‒white (WW) zones of freshly harvested human cadaver menisci and to characterize the vascularity of human menisci using immunofluorescence and 3-dimensional (3D) imaging. Fresh adult human menisci were harvested from healthy donors. Menisci were enzymatically digested, mononuclear cells isolated, and characterized using flow cytometry with antibodies against mesenchymal stem cell surface markers (CD105, CD90, CD44, and CD29). Cells were expanded in culture, characterized, and compared with bone marrow-derived mesenchymal stem cells. Trilineage differentiation potential of cultured cells was determined. Vasculature of menisci was mapped in 3D using a modified uDisco clearing and immunofluorescence against vascular markers CD31, lectin, and alpha smooth muscle actin. There were no significant differences in the clonogenicity of isolated cells between the 3 zones. Flow cytometry showed presence of CD44+CD105+CD29+CD90+ cells in all 3 zones with high prevalence in the WW zone. Progenitors from all zones were found to be potent to differentiate to mesenchymal lineages. Larger vessels in the red‒red zone of meniscus were observed spanning toward red‒white, sprouting to smaller arterioles and venules. CD31+ cells were identified in all zones using the 3D imaging and co-localization of additional markers of vasculature (lectin and alpha smooth muscle actin) was observed. The presence of resident mesenchymal progenitors was evident in all 3 meniscal zones of healthy adult donors without injury. In addition, our results demonstrate the presence of vascularization in the WW zone.
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生物学策略，以增强弯月面的血管区域的愈合。
影响因子：
4.3
作者：
Longo UG;Campi S;Romeo G;Spiezia F;Maffulli N;Denaro V
通讯作者：
Denaro V
影响因子：
1.7
作者：
Lu, Zhichao;Furumatsu, Takayuki;Ozaki, Toshifumi
通讯作者：
Ozaki, Toshifumi
影响因子：
2.4
作者：
Le Graverand, MPH;Ou, Y;Rattner, JB
通讯作者：
Rattner, JB
DOI：
10.1002/jor.23864
发表时间：
2018-07
期刊：
Journal of orthopaedic research : official publication of the Orthopaedic Research Society
影响因子：
--
作者：
Grogan SP;Duffy SF;Pauli C;Lotz MK;D'Lima DD
通讯作者：
D'Lima DD
影响因子：
2.6
作者：
Cinque, Mark E.;DePhillipo, Nicholas N.;LaPrade, Robert F.
通讯作者：
LaPrade, Robert F.