A complete mass-spectrometric map of the yeast proteome applied to quantitative trait analysis.
A complete mass-spectrometric map of the yeast proteome applied to quantitative trait analysis.
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DOI：
10.1038/nature11835
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发表时间：
2013-02-14
期刊：
影响因子：
64.8
通讯作者：
Aebersold, Ruedi
中科院分区：
文献类型：
--
作者：
Picotti, Paola;Clement-Ziza, Mathieu;Lam, Henry;Campbell, David S.;Schmidt, Alexander;Deutsch, Eric W.;Roest, Hannes;Sun, Zhi;Rinner, Oliver;Reiter, Lukas;Shen, Qin;Michaelson, Jacob J.;Frei, Andreas;Alberti, Simon;Kusebauch, Ulrike;Wollscheid, Bernd;Moritz, Robert L.;Beyer, Andreas;Aebersold, Ruedi
完整的参考图或数据集，如生物体的基因组图，对于生物和生物医学研究是非常有益的工具。到目前为止，为蛋白质组生成这样的参考数据集的尝试未能达到完全的蛋白质组覆盖，即使对于特征最彻底的蛋白质组，大约三分之二的蛋白质组也表现出饱和。在这里，我们使用了一种基于高通量肽合成和质谱仪的策略来生成接近完整的酿酒酵母蛋白质组参考图谱(97%的基因组预测蛋白质)。我们生成了这张质谱图的两个版本，一个支持发现--(猎枪)，另一个是假说驱动的(靶向)蛋白质组测量。因此，这两个版本的图谱构成了一套完整的蛋白质组分析，以支持使用当代蛋白质组技术进行的大多数研究。可以通过基于网络的储存库和相关的导航工具来浏览参考库。为了证明参考文库的实用性，我们将它们应用于蛋白质数量性状基因座(PQTL)分析，这需要在大量样本上高精度地测量相同的多肽。对一组78株酿酒酵母的蛋白质测量表明，独立的遗传位点之间存在复杂的关系，影响相关蛋白质的水平。我们的结果表明，选择性压力有利于获得维持蛋白质复合体和途径的化学计量比的一组多态。
Complete reference maps or datasets, like the genomic map of an organism, are highly beneficial tools for biological and biomedical research. Attempts to generate such reference datasets for a proteome so far failed to reach complete proteome coverage, with saturation apparent at approximately two thirds of the proteomes tested, even for the most thoroughly characterized proteomes. Here, we used a strategy based on high-throughput peptide synthesis and mass spectrometry to generate a close to complete reference map (97% of the genome-predicted proteins) of the S. cerevisiae proteome. We generated two versions of this mass spectrometric map one supporting discovery- (shotgun) and the other hypothesis-driven (targeted) proteomic measurements. The two versions of the map, therefore, constitute a complete set of proteomic assays to support most studies performed with contemporary proteomic technologies. The reference libraries can be browsed via a web-based repository and associated navigation tools. To demonstrate the utility of the reference libraries we applied them to a protein quantitative trait locus (pQTL) analysis, which requires measurement of the same peptides over a large number of samples with high precision. Protein measurements over a set of 78 S. cerevisiae strains revealed a complex relationship between independent genetic loci, impacting on the levels of related proteins. Our results suggest that selective pressure favors the acquisition of sets of polymorphisms that maintain the stoichiometry of protein complexes and pathways.
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影响因子：
9.9
作者：
Lange, Vinzenz;Picotti, Paola;Domon, Bruno;Aebersold, Ruedi
通讯作者：
Aebersold, Ruedi
影响因子：
3.2
作者：
de Smidt, Olga;du Preez, James C.;Albertyn, Jacobus
通讯作者：
Albertyn, Jacobus
影响因子：
3.7
作者：
Ackermann, Marit;Clement-Ziza, Mathieu;Beyer, Andreas
通讯作者：
Beyer, Andreas
影响因子：
48
作者：
Reiter, Lukas;Rinner, Oliver;Aebersold, Ruedi
通讯作者：
Aebersold, Ruedi
影响因子：
5.8
作者：
Craig, R;Beavis, RC
通讯作者：
Beavis, RC