Developmentally and spatially regulated expression of HNK-1 carbohydrate antigen on a novel phosphatidylinositol-anchored glycoprotein in rat brain
Developmentally and spatially regulated expression of HNK-1 carbohydrate antigen on a novel phosphatidylinositol-anchored glycoprotein in rat brain
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大鼠脑中新型磷脂酰肌醇锚定糖蛋白上 HNK-1 碳水化合物抗原的发育和空间调节表达
作者：
Y. Yoshihara;S. Oka;Y. Watanabe;K. Mori
HNK-1糖类抗原是一种表位，普遍表达于神经系统许多细胞表面的黏附和识别分子中。我们从大鼠脑中纯化并鉴定了一种新的磷脂酰肌醇(PI)锚定的150-kD糖蛋白，属于HNK-1家族。结合PI特异性磷脂酶C处理脑膜和用单抗HNK-1进行Western印迹分析，检测到PI-GP150分子。用三次层析(Sephacryl S-300、单抗HNK-1-Sepharose4B和Mono Q)从PI-PLC释放的材料中纯化了HNK-1阳性的PI-GP150，免疫印迹和结构分析证明是一个新的分子。抗PI-GP150的多克隆抗体被用来证明：(A)PI-GP150表达于培养的神经元细胞体及其突起的表面，以及(B)PI-GP150出现在胚胎发育过程中，并存在于所有脑区的整个出生后。然而，PI-GP150上的HNK-1表位的表达受到发育阶段特异性和区域特异性的调节。在新生大鼠中，HNK-1表位在PI-GP150上表达于整个大脑。PI-GP150上的HNK-1表位在出生后7天后在后脑中下降，在成人髓脑和后脑中完全缺失。而PI-GP150上的HNK-1表位在端脑中呈结构性表达。因此，虽然HNK-1碳水化合物表位与PI-GP150强烈偶联，但其表达可以独立于PI-GP150来调节。不同吻尾轴水平的HNK-1表位在脑膜其他HNK-1家族分子中的差异表达也被观察到。这些结果表明，HNK-1表位在对细胞表面分子的功能进行区域特异性和发育阶段特异性修饰方面起着重要作用。
HNK-1 carbohydrate antigen in an epitope expressed commonly in many cell surface adhesion and recognition molecules in the nervous system. We purified and characterized from rat brain a novel phosphatidylinositol (PI)-anchored 150-kD glycoprotein belonging to the HNK-1 family. The molecule (PI-GP150) was detected by combination of PI- specific phospholipase C treatment of brain membranes and Western blot analysis with mAb HNK-1. HNK-1-positive PI-GP150 was purified from the PI-PLC-released materials with three successive chromatographies (Sephacryl S-300, mAb HNK-1-Sepharose 4B, and Mono Q) and proven to be a novel molecule by immunoblot and structural analyses. Polyclonal antibody was raised against PI-GP150 and used to show that (a) PI-GP150 is expressed on the surface of neuronal cell bodies and their processes in culture, and (b) PI-GP150 appears during embryonic development and is present throughout all postnatal life in all brain regions. However, the expression of the HNK-1 epitope on PI-GP150 is regulated in both developmental stage-specific and region-specific manners. In newborn rats, the HNK-1 epitope is expressed on PI-GP150 throughout the brain. The level of HNK-1 epitope on PI-GP150 decreases after postnatal day 7 in hindbrain and becomes completely absent in adult myelencephalon and metencephalon. In contrast, HNK-1 epitope on PI-GP150 was constitutively expressed in telencephalon. Thus, while the HNK-1 carbohydrate epitope is strongly coupled to PI-GP150, its expression can be regulated independently of that of PI-GP150. The differential expression of the HNK-1 epitope at different rostro-caudal axial levels was observed also in other HNK-1 family molecules in brain membranes. These results suggest that the HNK-1 epitope plays an important role in adding region-specific and developmental stage-specific modifications on the function of the cell surface molecules.
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影响因子：
56.9
作者：
CUNNINGHAM, BA;HEMPERLY, JJ;EDELMAN, GM
通讯作者：
EDELMAN, GM
含硫酸化葡萄糖醛酸的糖复合物是发育中的哺乳动物神经系统中的时间和空间调节抗原。
影响因子：
2.7
作者：
Schwarting,GA;Jungalwala,FB;Chou,DK;Boyer,AM;Yamamoto,M
通讯作者：
Yamamoto,M
神经病患者中含有糖脂的硫酸化葡萄糖醛酸与 IgM M 蛋白反应的表征。
DOI：
--
发表时间：
1987
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Ariga,T;Kohriyama,T;Freddo,L;Latov,N;Saito,M;Kon,K;Ando,S;Suzuki,M;Hemling,ME;RinehartJr,KL
通讯作者：
RinehartJr,KL
通过单克隆抗体 (HNK-1) 识别的人类 NK 和 K 细胞的分化抗原。
DOI：
--
发表时间：
1981
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Abo,T;Balch,CM
通讯作者：
Balch,CM
神经系统中硫酸化葡萄糖醛酸糖脂的结构与神经病中的 HNK-1 抗体和一些 IgM 副蛋白发生反应。
DOI：
--
发表时间：
1986
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Chou,DK;Ilyas,AA;Evans,JE;Costello,C;Quarles,RH;Jungalwala,FB
通讯作者：
Jungalwala,FB