Inflammatory Endotypes and Tissue Remodeling Features in Antrochoanal Polyps.
Inflammatory Endotypes and Tissue Remodeling Features in Antrochoanal Polyps.
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后鼻孔息肉的炎症内型和组织重塑特征
DOI：
10.4168/aair.2021.13.6.863
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发表时间：
2021-11
期刊：
影响因子：
--
通讯作者：
Liu Z
中科院分区：
文献类型：
--
作者：
Chen CL;Wang YT;Yao Y;Pan L;Guo B;Zhu KZ;Ma J;Wang N;Li XL;Deng YK;Liu Z
目的上颌窦后鼻孔息肉（antrochoanal polyps，ACP）的发病机制尚不清楚。本研究旨在描述ACP的炎症模式和组织重塑特征。方法采用免疫组化和免疫荧光染色方法，观察ACP、双侧嗜酸性和非嗜酸性鼻息肉（NPs）中炎性细胞浸润、组织水肿程度和纤维蛋白沉积情况。采用Bio-ELISA法检测鼻腔鼻窦组织中细胞因子水平。采用逆转录聚合酶链反应和酶联免疫吸附试验测定凝血和纤溶标志物的表达。结果与对照组及双侧嗜酸性粒细胞和非嗜酸性粒细胞NPs相比，ACP组中性粒细胞浸润及髓过氧化物酶（MPO）、白细胞介素（IL）-8和干扰素（IFN）-γ的表达均明显增高。总的来说，94.4%的ACP表现出嗜酸性粒细胞阳离子蛋白/MPO比值< 1，相比之下，79.0%的非嗜酸性粒细胞和26%的嗜酸性粒细胞NP。主成分分析和多重对应分析显示ACP中的嗜中性粒细胞和1型炎症模式。与对照组织相比，水肿评分和纤维蛋白沉积增加，而d-二聚体和组织纤溶酶原激活物（tPA）水平降低，ACP的变化甚至比嗜酸性粒细胞NP更突出。ACP患者tPA水平与IFN-γ、IL-8、MPO水平呈负相关。中性粒细胞是ACP中IFN-γ的主要细胞来源，ACP中IFN-γ+中性粒细胞的数量高于对照组织和双侧嗜酸性和非嗜酸性NPs。结论ACP具有嗜酸性和1型炎症内型特征。中性粒细胞来源的IFN-γ与ACP中tPA产生减少相关。
Purpose The pathogenic mechanisms of antrochoanal polyps (ACPs) remain largely unknown. This study aimed to characterize inflammatory patterns and tissue remodeling features in ACPs. Methods Inflammatory cell infiltration and tissue edema severity as well as fibrin deposition in ACPs and bilateral eosinophilic and noneosinophilic nasal polyps (NPs) were studied with immunohistochemical and immunofluorescence staining. Cytokine levels in sinonasal tissues were detected with the Bio-Plex assay. The expression of coagulation and fibrinolytic markers was measured using reverse-transcription polymerase chain reaction and enzyme-linked immunosorbent assays. Results Compared to control tissues and bilateral eosinophilic and noneosinophilic NPs, ACPs had higher levels of neutrophil infiltration and expression of myeloperoxidase (MPO), interleukin (IL)-8 and interferon (IFN)-γ. In total, 94.4% of ACPs demonstrated an eosinophil cationic protein/MPO ratio of < 1, compared to 79.0% of noneosinophilic and 26% of eosinophilic NPs. Principle component and multiple correspondence analyses revealed a neutrophilic and type 1 inflammation pattern in ACPs. Compared to control tissues, edema scores and fibrin deposition were increased, whereas d-dimer and tissue plasminogen activator (tPA) levels were decreased in ACPs and bilateral NPs, with more prominent changes in ACPs even than in eosinophilic NPs. The tPA levels were negatively correlated with IFN-γ, IL-8, and MPO levels in ACPs. Neutrophils were the major cellular source of IFN-γ in ACPs, and the number of IFN-γ+ neutrophils was elevated in ACPs than in control tissues and bilateral eosinophilic and noneosinophilic NPs. Conclusions ACPs are characterized by the neutrophilic and type 1 inflammation endotype. Neutrophil-derived IFN-γ is associated with reduced tPA production in ACPs.
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影响因子：
7.2
作者：
Fokkens, Wytske J.;Lund, Valerie J.;Zwetsloot, Casper P.
通讯作者：
Zwetsloot, Casper P.
影响因子：
2.6
作者：
Atighechi, Saeid;Baradaranfar, Mohammad Hossein;Jafari, Rozita
通讯作者：
Jafari, Rozita
影响因子：
14.2
作者：
Bachert, Claus;Gevaert, Elien
通讯作者：
Gevaert, Elien
DOI：
10.1111/cea.12304
发表时间：
2014
期刊：
Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology
影响因子：
--
作者：
Cao PP;Zhang YN;Liao B;Ma J;Wang BF;Wang H;Zeng M;Liu WH;Schleimer RP;Liu Z
通讯作者：
Liu Z
影响因子：
6.4
作者：
Orlandi, Richard R.;Kingdom, Todd T.;Hwang, Peter H.
通讯作者：
Hwang, Peter H.