Increased local IgE production induced by common aeroallergens and phenotypic alteration of mast cells in Chinese eosinophilic, but not non-eosinophilic, chronic rhinosinusitis with nasal polyps.
Increased local IgE production induced by common aeroallergens and phenotypic alteration of mast cells in Chinese eosinophilic, but not non-eosinophilic, chronic rhinosinusitis with nasal polyps.
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DOI：
10.1111/cea.12304
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Liu Z
中科院分区：
文献类型：
--
作者：
Cao PP;Zhang YN;Liao B;Ma J;Wang BF;Wang H;Zeng M;Liu WH;Schleimer RP;Liu Z
嗜酸性和非嗜酸性慢性鼻窦炎鼻息肉（CRSwNP）显示不同的炎症模式。然而，CRSwNP异质性的致病机制需要进一步研究。研究嗜酸性粒细胞和非嗜酸性粒细胞CRSwNP中肥大细胞的表型和局部免疫球蛋白E（IgE）的产生。通过ImmunoCAP系统分析总IgE和特异性IgE水平。使用RT-PCR测定研究了类转换重组至IgE的分子步骤。采用免疫组化方法检测鼻腔黏膜肥大细胞表型、IgE和高亲和力IgE受体（FcεRI）阳性细胞以及过敏原与特异性IgE的结合。与对照组和非嗜酸性粒细胞CRSwNP相比，在中国嗜酸性粒细胞CRSwNP中，局部总IgE水平升高，并且更频繁地发现对常见空气过敏原的局部特异性IgE，与特应性无关，并且与金黄色葡萄球菌肠毒素无显著相关性。在嗜酸性粒细胞CRSwNP中也更频繁地检测到ε生殖系基因转录物。嗜酸性粒细胞CRSwNP中IgE和Fcε RI阳性细胞的数量增加。IgE和Fcε RI阳性细胞多为肥大细胞。尘螨抗原可与肥大细胞表面的IgE原位结合。嗜酸性粒细胞CRSwNP中类胰蛋白酶和糜蛋白酶阳性的肥大细胞和活化肥大细胞的数量增加，活化肥大细胞的数量与CRSwNP中局部IgE水平、嗜酸性粒细胞趋化因子-1水平和嗜酸性粒细胞计数呈正相关。常见的吸入性变应原诱导的局部IgE可能介导肥大细胞活化，并导致中国人CRSwNP中随后的嗜酸性炎症。这项研究提供了一个合理的考虑干预策略，旨在针对嗜酸性粒细胞CRSwNP的“局部过敏”。
Eosinophilic and non-eosinophilic chronic rhinosinusitis with nasal polyps (CRSwNP) display distinct patterns of inflammation. However, the pathogenic mechanisms underlying the heterogeneity of CRSwNP need further investigation. To investigate local immunoglobulin E (IgE) production and phenotype of mast cells in eosinophilic and non-eosinophilic CRSwNP in Chinese. Total and specific IgE levels were analyzed by means of the ImmunoCAP system. The molecular steps involved in class switch recombination to IgE were investigated using RT-PCR assays. Mast cell phenotypes, IgE- and high affinity IgE receptor (FcεRI)-positive cells, and allergen binding to specific IgE in sinonasal mucosa were determined by means of immunohistochemistry. Compared with controls and non-eosinophilic CRSwNP, local total IgE levels were increased, and local specific IgE to common aeroallergens was more frequently found, in Chinese eosinophilic CRSwNP independent of atopy and without significant association with Staphylococcus aureus enterotoxins. The ε germline gene transcript was also more frequently detected in eosinophilic CRSwNP. The number of IgE- and FcεRI-positive cells was increased in eosinophilic CRSwNP. Most IgE- and FcεRI-positive cells were mast cells. Dust mite antigens could bind to IgE on mast cells in situ. The number of mast cells positive for both tryptase and chymase and activated mast cells was increased in eosinophilic CRSwNP and the number of activated mast cells positively correlated with local IgE level, eotaxin-1 level, and eosinophil count in CRSwNP. The local IgE induced by common aeroallergens may mediate mast cell activation and contribute to subsequent eosinophilic inflammation in Chinese CRSwNP. This study offers a rationale for considering intervention strategies designed to target “local allergy” in eosinophilic CRSwNP.
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影响因子：
14.2
作者：
Abonia, J. Pablo;Blanchard, Carine;Butz, Bridget Buckmeier;Rainey, Heather F.;Collins, Margaret H.;Stringer, Keith;Putnam, Philip E.;Rothenberg, Marc E.
通讯作者：
Rothenberg, Marc E.
DOI：
10.1016/j.jaci.2012.02.046
发表时间：
2012-08
期刊：
The Journal of allergy and clinical immunology
影响因子：
--
作者：
Takabayashi T;Kato A;Peters AT;Suh LA;Carter R;Norton J;Grammer LC;Tan BK;Chandra RK;Conley DB;Kern RC;Fujieda S;Schleimer RP
通讯作者：
Schleimer RP
影响因子：
12.4
作者：
Liu, Z.;Lu, X.;Cui, Y. H.
通讯作者：
Cui, Y. H.
影响因子：
14.2
作者：
Bachert, C;Gevaert, P;van Cauwenberge, P
通讯作者：
van Cauwenberge, P
影响因子：
4.4
作者：
Takhar, P;Smurthwaite, L;Gould, HJ
通讯作者：
Gould, HJ