Toward Point-of-Care Detection of Mycobacterium tuberculosis: A Brighter Solvatochromic Probe Detects Mycobacteria within Minutes.
Toward Point-of-Care Detection of Mycobacterium tuberculosis: A Brighter Solvatochromic Probe Detects Mycobacteria within Minutes.
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DOI：
10.1021/jacsau.1c00173
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发表时间：
2021-09-27
期刊：
影响因子：
8
通讯作者：
Bertozzi CR
中科院分区：
文献类型：
--
作者：
Kamariza M;Keyser SGL;Utz A;Knapp BD;Ealand C;Ahn G;Cambier CJ;Chen T;Kana B;Huang KC;Bertozzi CR
迫切需要快速、廉价和操作简单的结核病(TB)诊断方法。在这里，我们报告了一种明亮的溶剂变色染料海藻糖结合物，它能在几分钟内特异性地检测结核分枝杆菌(Mtb)。3-羟基色酮(3HC)染料具有高的荧光量子产率，随着环境极性的变化，其荧光强度发生变化。我们合成了两个3HC-海藻糖结合物的类似物(3HC-2-tre和3HC-3-tre)，并确定3HC-3-tre对结核分枝杆菌具有非常好的检测性能。3HC-3-TrE标记的分枝杆菌细胞的荧光强度比我们之前报道的4，4-N，N-二甲氨基邻苯二甲酰亚胺(DMN-TrE)的荧光强度增加了10倍。令人兴奋的是，我们在探针处理后的10分钟内检测到了荧光结核分枝杆菌细胞。因此，3HC-3-TrE允许快速可视化分枝杆菌，最终可以在资源不足的情况下改进护理点的结核分枝杆菌检测。
There is an urgent need for point-of-care tuberculosis (TB) diagnostic methods that are fast, inexpensive, and operationally simple. Here, we report on a bright solvatochromic dye trehalose conjugate that specifically detects Mycobacterium tuberculosis (Mtb) in minutes. 3-Hydroxychromone (3HC) dyes, known for having high fluorescence quantum yields, exhibit shifts in fluorescence intensity in response to changes in environmental polarity. We synthesized two analogs of 3HC-trehalose conjugates (3HC-2-Tre and 3HC-3-Tre) and determined that 3HC-3-Tre has exceptionally favorable properties for Mtb detection. 3HC-3-Tre-labeled mycobacterial cells displayed a 10-fold increase in fluorescence intensity compared to our previous reports on the dye 4,4-N,N-dimethylaminonapthalimide (DMN-Tre). Excitingly, we detected fluorescent Mtb cells within 10 min of probe treatment. Thus, 3HC-3-Tre permits rapid visualization of mycobacteria that ultimately could empower improved Mtb detection at the point-of-care in low-resource settings.
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影响因子：
15
作者：
Rodriguez-Rivera FP;Zhou X;Theriot JA;Bertozzi CR
通讯作者：
Bertozzi CR
影响因子：
14.8
作者：
Backus, Keriann M.;Boshoff, Helena L.;Barry, Conor S.;Boutureira, Omar;Patel, Mitul K.;D'Hooge, Francois;Lee, Seung Seo;Via, Laura E.;Tahlan, Kapil;Barry, Clifton E., III;Davis, Benjamin G.
通讯作者：
Davis, Benjamin G.
影响因子：
17.1
作者：
Kamariza M;Shieh P;Ealand CS;Peters JS;Chu B;Rodriguez-Rivera FP;Babu Sait MR;Treuren WV;Martinson N;Kalscheuer R;Kana BD;Bertozzi CR
通讯作者：
Bertozzi CR
影响因子：
5.2
作者：
Gunduz, Simay;Goren, Ahmet C.;Ozturk, Turan
通讯作者：
Ozturk, Turan
影响因子：
5.7
作者：
Kucherak, Oleksandr A.;Didier, Pascal;Klymchenko, Andrey S.
通讯作者：
Klymchenko, Andrey S.