The effect of Cd on mycorrhizal development and enzyme activity of Glomus mosseae and Glomus intraradices in Astragalus sinicus L.
The effect of Cd on mycorrhizal development and enzyme activity of Glomus mosseae and Glomus intraradices in Astragalus sinicus L.
复制标题
镉对黄芪苔藓球囊霉和根内球囊霉菌根发育及酶活性的影响。
DOI：
10.1016/j.chemosphere.2009.01.046
复制
发表时间：
2009-05
期刊：
影响因子：
8.8
通讯作者：
Peng, Jin
中科院分区：
文献类型：
--
作者：
Shi, Ping;Zhao, Bin;Li, Yan;Peng, Jin
研究了镉(Cd)对两种丛枝菌根真菌(AMF)根部定植的影响。以紫云英为试材，在土壤中加入四种浓度的Cd，分别接种苔藓球菌、球囊霉菌，或两种真菌混合接种。7周后用组织化学活体染色检测菌根共生的效率和功能。同时应用碱性磷酸酶活性根染色的巢式聚合酶链式反应监测混合群落的丛枝菌根真菌效率。Cd的加入使植物生物量和菌根定殖率略有下降，碱性磷酸酶(ALP)和琥珀酸脱氢酶(SDH)的活性也有所降低，其中以海藻的抑制最为明显。无论Cd的添加水平如何，菌根定植都显着促进了植物的生长。接种病原菌或混合菌剂的植株对Cd在根中的固定较多，在Cd添加水平最高时，向地上部分配的Cd较少。在混合群落中，海绵的定殖率得到了提高，苔藓海绵是该群落的优势定殖者。基于该菌株对Cd的耐性较高，因此该菌株是Cd污染土壤生物修复的首选菌株。
The influence of cadmium (Cd) on the root colonization of Astragalus by two arbuscular mycorrhizal fungi (AMF) was investigated. Astragalus sinicus L. grown in the soil in the presence of four levels of Cd was inoculated individually with Glomus mosseae, Glomus intraradices, or by a mixed inoculum of these two fungi. Histochemical vital stains were used to measure the efficiency and function of the mycorrhizal symbiosis after 7weeks. Nested PCR with ALP-active stained roots was applied to monitor the efficiency AMF in mixed community at the same time. The addition of Cd slightly decreased plant biomass and mycorrhizal colonization, and depressed the activities of alkaline phosphatase (ALP) and succinate dehydrogenase (SDH), especially in G. intraradices. Mycorrhizal colonization significantly improved plant growth regardless of the level of addition of Cd. The plants inoculated with G. mosseae or mixed inoculum immobilized more Cd in the roots, and partitioned less Cd to the shoots at the highest Cd addition level. Root colonization efficiency of G. intraradices was improved in the mixed community and G. mosseae was the dominant colonizer in this community. Based on the higher Cd tolerance of this strain of G. mosseae, it would be preferred for bioremediation in Cd contaminated soil.
登录
查看更多内容
影响因子：
4.9
作者：
E. Jacquot;D. V. Tuinen;S. Gianinazzi;V. Gianinazzi‐Pearson
通讯作者：
E. Jacquot;D. V. Tuinen;S. Gianinazzi;V. Gianinazzi‐Pearson
影响因子：
4.4
作者：
C. Val;J. Barea;C. Azcón-Aguilar
通讯作者：
C. Val;J. Barea;C. Azcón-Aguilar
DOI：
10.1201/9781420039993.ch8
发表时间：
2000-09
期刊：
--
影响因子：
--
作者：
C. Leyval;E. Joner
通讯作者：
C. Leyval;E. Joner
影响因子：
17.3
作者：
H. Seliger
通讯作者：
H. Seliger
影响因子：
3.9
作者：
Chen, BD;Liu, Y;Christie, P
通讯作者：
Christie, P