Uncovering the molecular mechanisms of lignocellulose digestion in shipworms.
Uncovering the molecular mechanisms of lignocellulose digestion in shipworms.
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DOI：
10.1186/s13068-018-1058-3
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发表时间：
2018
影响因子：
6.3
通讯作者：
McQueen-Mason SJ
中科院分区：
文献类型：
--
作者：
Sabbadin F;Pesante G;Elias L;Besser K;Li Y;Steele-King C;Stark M;Rathbone DA;Dowle AA;Bates R;Shipway JR;Cragg SM;Bruce NC;McQueen-Mason SJ
木质纤维素形成木本植物生物质的结构框架，并且代表生物圈中最丰富的碳源。木质生物质的周转是全球碳循环的关键组成部分，随着工业从化石燃料转向可再生碳资源，所涉及的酶在工业上的重要性日益增加。船虫是海洋双壳类软体动物，消化木材，通过加工海洋中的植物生物质在全球碳循环中发挥关键作用。以往的研究表明，在船蛆的木材消化是由内共生细菌在动物的鳃中发现的酶为主，而鲜为人知的是船蛆产生的内源性酶的身份和功能。使用元转录组学，蛋白质组学，成像和生物化学分析的组合，我们揭示了一个复杂的消化系统占主导地位的一个专门的消化腺分泌的未知酶，并积累在盲肠，木材消化发生。使用转录组学，蛋白质组学和显微镜相结合，我们表明，消化蛋白质组的船虫Lyrodus pedicellatus主要是由动物本身产生的酶，与共生细菌的一个小的，但显着的贡献。消化蛋白质组主要由一种新的300 kDa多结构域糖苷水解酶控制，该水解酶在β-1，4-葡聚糖（木材中最丰富的聚合物）的水解中起作用。这些研究使人们对海洋环境中木材回收利用的一个不寻常的、具有生态重要性的过程有了前所未有的深入了解，并为从木质纤维素生物质中动员糖开辟了新的生物技术机会。本文的在线版本（10.1186/s13068-018-1058-3）包含补充材料，可供授权用户使用。
Lignocellulose forms the structural framework of woody plant biomass and represents the most abundant carbon source in the biosphere. Turnover of woody biomass is a critical component of the global carbon cycle, and the enzymes involved are of increasing industrial importance as industry moves away from fossil fuels to renewable carbon resources. Shipworms are marine bivalve molluscs that digest wood and play a key role in global carbon cycling by processing plant biomass in the oceans. Previous studies suggest that wood digestion in shipworms is dominated by enzymes produced by endosymbiotic bacteria found in the animal’s gills, while little is known about the identity and function of endogenous enzymes produced by shipworms. Using a combination of meta-transcriptomic, proteomic, imaging and biochemical analyses, we reveal a complex digestive system dominated by uncharacterized enzymes that are secreted by a specialized digestive gland and that accumulate in the cecum, where wood digestion occurs. Using a combination of transcriptomics, proteomics, and microscopy, we show that the digestive proteome of the shipworm Lyrodus pedicellatus is mostly composed of enzymes produced by the animal itself, with a small but significant contribution from symbiotic bacteria. The digestive proteome is dominated by a novel 300 kDa multi-domain glycoside hydrolase that functions in the hydrolysis of β-1,4-glucans, the most abundant polymers in wood. These studies allow an unprecedented level of insight into an unusual and ecologically important process for wood recycling in the marine environment, and open up new biotechnological opportunities in the mobilization of sugars from lignocellulosic biomass. The online version of this article (10.1186/s13068-018-1058-3) contains supplementary material, which is available to authorized users.
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DOI：
10.1073/pnas.1620470114
发表时间：
2017-05-02
影响因子：
11.1
作者：
Distel, Daniel L.;Altamia, Marvin A.;Haygood, Margo G.
通讯作者：
Haygood, Margo G.
影响因子：
7
作者：
Ishihama, Y;Oda, Y;Mann, M
通讯作者：
Mann, M
影响因子：
4.4
作者：
Dowle, Adam A.;Wilson, Julie;Thomas, Jerry R.
通讯作者：
Thomas, Jerry R.
影响因子：
4.8
作者：
Jacob, R;Peters, K;Naim, HY
通讯作者：
Naim, HY
影响因子：
5.2
作者：
Franco Cairo JP;Carazzolle MF;Leonardo FC;Mofatto LS;Brenelli LB;Gonçalves TA;Uchima CA;Domingues RR;Alvarez TM;Tramontina R;Vidal RO;Costa FF;Costa-Leonardo AM;Paes Leme AF;Pereira GA;Squina FM
通讯作者：
Squina FM