Expanding the Knowledge on Lignocellulolytic and Redox Enzymes of Worker and Soldier Castes from the Lower Termite Coptotermes gestroi.
Expanding the Knowledge on Lignocellulolytic and Redox Enzymes of Worker and Soldier Castes from the Lower Termite Coptotermes gestroi.
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DOI：
10.3389/fmicb.2016.01518
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发表时间：
2016
影响因子：
5.2
通讯作者：
Squina FM
中科院分区：
文献类型：
--
作者：
Franco Cairo JP;Carazzolle MF;Leonardo FC;Mofatto LS;Brenelli LB;Gonçalves TA;Uchima CA;Domingues RR;Alvarez TM;Tramontina R;Vidal RO;Costa FF;Costa-Leonardo AM;Paes Leme AF;Pereira GA;Squina FM
白蚁被认为是地球上木质纤维素最有效的分解者之一，因为它们能够与其微生物共生体沿着产生碳水化合物活性酶（CAZymes）的全部功能。近年来，白蚁体内的一系列促氧化酶、抗氧化酶和脱氢酶（PAD）也与糖类和木质素的代谢有关。低等白蚁Coptotermes gestroi被认为是巴西主要的城市害虫，对木结构造成破坏。最近，对C. gestroi揭开了不同CAZymes的存在。由于C. gestroi和它们的共生原生生物的关系尚不清楚，本研究的目的是探索这些酶在C.管理员我们的研究结果表明，工人和士兵种姓呈现出相似的CAZy/PAD酶谱，并且还证实了内切葡聚糖酶（GH 9）和β-葡糖苷酶（GH 1）是两种种姓中与木质纤维素降解相关的最重要的糖苷水解酶家族。经典的纤维素酶如外切葡聚糖酶（GH 7）和内切葡聚糖酶（GH5和GH45）以及经典的木聚糖酶（GH 10和GH 11）仅在分类学上与原生生物相关的两种种姓中发现，突出了共生在C.管理员此外，我们的分析揭示了辅助活性酶家族（AAs）的存在，这可能与白蚁气孔中的木质素修饰有关。总之，该报告扩展了对来自较低白蚁的工人和士兵种姓的与CAZy/PAD酶相关的基因和蛋白质的了解，揭示了用于第二代生物燃料过程的新的潜在酶候选物。
Termites are considered one of the most efficient decomposers of lignocelluloses on Earth due to their ability to produce, along with its microbial symbionts, a repertoire of carbohydrate-active enzymes (CAZymes). Recently, a set of Pro-oxidant, Antioxidant, and Detoxification enzymes (PAD) were also correlated with the metabolism of carbohydrates and lignin in termites. The lower termite Coptotermes gestroi is considered the main urban pest in Brazil, causing damage to wood constructions. Recently, analysis of the enzymatic repertoire of C. gestroi unveiled the presence of different CAZymes. Because the gene profile of CAZy/PAD enzymes endogenously synthesized by C. gestroi and also by their symbiotic protists remains unclear, the aim of this study was to explore the eukaryotic repertoire of these enzymes in worker and soldier castes of C. gestroi. Our findings showed that worker and soldier castes present similar repertoires of CAZy/PAD enzymes, and also confirmed that endo-glucanases (GH9) and beta-glucosidases (GH1) were the most important glycoside hydrolase families related to lignocellulose degradation in both castes. Classical cellulases such as exo-glucanases (GH7) and endo-glucanases (GH5 and GH45), as well as classical xylanases (GH10 and GH11), were found in both castes only taxonomically related to protists, highlighting the importance of symbiosis in C. gestroi. Moreover, our analysis revealed the presence of Auxiliary Activity enzyme families (AAs), which could be related to lignin modifications in termite digestomes. In conclusion, this report expanded the knowledge on genes and proteins related to CAZy/PAD enzymes from worker and soldier castes of lower termites, revealing new potential enzyme candidates for second-generation biofuel processes.
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影响因子：
5.2
作者：
Deangelis KM;Sharma D;Varney R;Simmons B;Isern NG;Markilllie LM;Nicora C;Norbeck AD;Taylor RC;Aldrich JT;Robinson EW
通讯作者：
Robinson EW
DOI：
10.1073/pnas.0805257105
发表时间：
2008-09-02
影响因子：
11.1
作者：
Geib, Scott M.;Filley, Timothy R.;Tien, Ming
通讯作者：
Tien, Ming
影响因子：
5.8
作者：
Larkin, M. A.;Blackshields, G.;Higgins, D. G.
通讯作者：
Higgins, D. G.
影响因子：
11
作者：
Burnum, Kristin E.;Callister, Stephen J.;Lipton, Mary S.
通讯作者：
Lipton, Mary S.
影响因子：
3.8
作者：
Coy, M. R.;Salem, T. Z.;Scharf, M. E.
通讯作者：
Scharf, M. E.