Putative papain-related thiol proteases of positive-strand RNA viruses. Identification of rubi- and aphthovirus proteases and delineation of a novel conserved domain associated with proteases of rubi-, alpha- and coronaviruses.
Putative papain-related thiol proteases of positive-strand RNA viruses. Identification of rubi- and aphthovirus proteases and delineation of a novel conserved domain associated with proteases of rubi-, alpha- and coronaviruses.
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DOI：
10.1016/0014-5793(91)81034-6
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发表时间：
1991-08-19
期刊：
影响因子：
3.5
通讯作者：
Lai MM
中科院分区：
文献类型：
--
作者：
Gorbalenya AE;Koonin EV;Lai MM
计算机辅助比较分析了由几个不同群体或正链RNA病毒的基因组编码的（推定的）巯基蛋白酶的氨基酸序列，这些巯基蛋白酶与细胞木瓜蛋白酶样蛋白酶家族有较远的关系。在口蹄疫病毒的前导蛋白酶和小鼠肝炎冠状病毒的蛋白酶之间检测到高度相似性，该蛋白酶从多聚蛋白切割N末端p28蛋白。获得了风疹病毒多聚蛋白的一部分与细胞木瓜蛋白酶样蛋白酶的统计学显著性比对，从而初步鉴定木瓜蛋白酶样蛋白酶是介导该病毒非结构蛋白加工的酶。揭示了α病毒、马铃薯Y病毒和大麦花叶病毒的蛋白酶序列之间的特异性分组。值得注意的是，正链RNA病毒的木瓜蛋白酶样蛋白酶在其序列和基因组位置上比在同一病毒类别中发现的糜蛋白酶相关蛋白酶更可变。还发现了一个新的未知功能的保守结构域，其位于α-、鲁比-和冠状病毒的木瓜蛋白酶样蛋白酶的侧翼。
A computer‐assisted comparative analysis of the amino acid sequences of (putative) thiol proteases encoded by the genomes of several diverse groups or positive‐stranded RNA viruses and distantly related to the family of cellular papain‐like proteases is presented. A high level of similarity was detected between the leader protease of foot‐and‐mouth‐disease virus and the protease of murine hepatitis coronavirus which cleaves the N‐terminal p28 protein from the polyprotein. Statistically significant alignment of a portion of the rubella virus polyprotein with cellular papain‐like proteases was obtained, leading to tentative identification of the papain‐like protease as the enzyme mediating processing of the non‐structural proteins of this virus. Specific grouping between the sequences of the proteases of α‐viruses, and poty‐ and bymoviruses was revealed. It was noted that papain‐like proteases of positive‐stranded RNA viruses are much more variable both in their sequences and in genomic locations than chymotrypsin‐related proteases found in the same virus class. A novel conserved domain of unknown function has also been identified which flanks the papain‐like proteases of α‐, rubi‐ and coronaviruses.
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影响因子：
14.9
作者：
Gorbalenya AE;Koonin EV;Donchenko AP;Blinov VM
通讯作者：
Blinov VM
影响因子：
3.5
作者：
GORBALENYA, AE;DONCHENKO, AP;KOONIN, EV
通讯作者：
KOONIN, EV
影响因子：
3.7
作者：
Dominguez G;Wang CY;Frey TK
通讯作者：
Frey TK
影响因子：
3.9
作者：
Gorbalenya AE;Blinov VM;Donchenko AP;Koonin EV
通讯作者：
Koonin EV
DOI：
10.1073/pnas.85.21.7872
发表时间：
1988-11-01
影响因子：
11.1
作者：
BAZAN, JF;FLETTERICK, RJ
通讯作者：
FLETTERICK, RJ