Sequence of the genome RNA of rubella virus: evidence for genetic rearrangement during togavirus evolution.
Sequence of the genome RNA of rubella virus: evidence for genetic rearrangement during togavirus evolution.
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DOI：
10.1016/0042-6822(90)90476-8
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发表时间：
1990-07
期刊：
影响因子：
3.7
通讯作者：
Frey TK
中科院分区：
文献类型：
--
作者：
Dominguez G;Wang CY;Frey TK
测定了风疹病毒（RUB）基因组RNA的核苷酸序列。RUB基因组RNA长度为9757个核苷酸（不包括聚(A)尾），G/C含量为69.5%，是迄今为止测序的RNA病毒中最高的。RUB基因组RNA包含两个长开放阅读框（ORF），一个5 ' -近端ORF包含6656个核苷酸，一个3 ' -近端ORF包含3189个核苷酸，用于编码结构蛋白。因此，RUB的基因组组织与托加病毒家族的另一个属甲型病毒相似，因此假定RUB的5 ' -近端ORF编码非结构蛋白。在RUB基因组RNA中发现了与甲病毒高度保守的3个核苷酸序列同源的序列（基因组5′端有一个茎环结构，基因组5′端有一个51个核苷酸的保守序列，亚基因组RNA起始位点有一个20个核苷酸的保守序列）。RUB与甲型病毒的非结构ORF氨基酸序列比较发现，只有一个短的（122个氨基酸）区域具有显著的同源性，表明这两种病毒仅是远亲。该同源区域位于甲病毒基因组nsP3的NH2末端。RUB非结构蛋白ORF包含两个全局氨基酸基序，在大量正极性RNA病毒中保守，一个基序指示解旋酶活性，一个基序指示复制酶活性。RUB基因组中解旋酶基序和nsP3同源区域的顺序与甲病毒基因组相反，表明在这些病毒的进化过程中发生了遗传重排。
The nucleotide sequence of the rubella virus (RUB) genomic RNA was determined. The RUB genomic RNA is 9757 nucleotides in length [excluding the poly(A) tail] and has a G/C content of 69.5%, the highest of any RNA virus sequenced to date. The RUB genomic RNA contains two long open reading frames (ORFs), a 5′-proximal ORF of 6656 nucleotides and a 3′-proximal ORF of 3189 nucleotides which encodes the structural proteins. Thus, the genomic organization of RUB is similar to that of alphaviruses, the other genus of the Togavirus family, and the 5′-proximal ORF of RUB therefore putatively codes for the nonstructural proteins. Sequences homologous to three regions of nucleotide sequence highly conserved among alphaviruses (a stem-and-loop structure at the 5′ end of the genome, a 51-nucleotide conserved sequence near the 5′ end of the genome, and a 20-nucleotide conserved sequence at the subgenomic RNA start site) were found in the RUB genomic RNA. Amino acid sequence comparisons between the nonstructural ORF of RUB and alphaviruses revealed only one short (122 amino acids) region of significant homology, indicating that these viruses are only distantly related. This region of homology is located at the NH2 terminus of nsP3 in the alphavirus genome. The RUB nonstructural protein ORF contains two global amino acid motifs conserved in a large number of positive-polarity RNA viruses, a motif indicative of helicase activity and a motif indicative of replicase activity. The order of the helicase motif and the nsP3 homology region in the RUB genome is reversed with respect to the alphavirus genome indicating that a genetic rearrangement has occurred during the evolution of these viruses.
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影响因子：
14.9
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