Evolutionary Quantitative Proteomics of Reproductive Protein Divergence in Drosophila.
Evolutionary Quantitative Proteomics of Reproductive Protein Divergence in Drosophila.
复制标题
DOI：
10.1016/j.mcpro.2023.100610
复制
发表时间：
2023-08
影响因子：
7
通讯作者：
Ahmed-Braimah, Yasir H.
中科院分区：
文献类型：
--
作者：
Garlovsky, Martin D.;Ahmed-Braimah, Yasir H.
繁殖特征在物种间往往进化得很快。要理解这种快速分化的原因和后果，就需要对女性和男性生殖蛋白及其对受精成功的影响进行表征。果蝇的种间生殖不亲和性很强，这使得它们成为研究生殖蛋白多样化及其在物种形成中的作用的理想选择。重要的是，射精内蛋白丰度和分配在种间分化中的作用尚不清楚。在这里，我们使用三种雄性阳痿组在交配前和交配后立即对雌性下生殖道进行多重等压标记，鉴定和量化了转移的雄性射精蛋白质组。我们鉴定了超过200种假定的雄性射精蛋白，其中许多在物种之间表现出不同的丰度，这表明雄性在交配过程中转移了一种特定物种的精液蛋白分配。我们还发现了超过2000种雌性生殖蛋白，它们含有雌性特有的丝氨酸型内多肽酶，在物种之间表现出不同的丰度和分子进化速度的提高，类似于一些雄性精液蛋白。我们的研究结果表明，生殖蛋白的差异也可以表现在物种特异性蛋白丰度模式上。我们同时描述了男性和女性生殖蛋白质组。我们鉴定了bbb2000个射精蛋白和bbb2000个女性生殖道蛋白。许多蛋白质显示出物种特有的丰度。差异丰富的蛋白质包括许多属于多拷贝基因家族的蛋白质。雌性丝氨酸型内肽酶的丰度不同，且进化迅速。两性之间的分子相互作用对受精成功至关重要。然而，相互作用的生殖性状的共同进化可能导致种群之间的生化不匹配和交配后的前合子隔离的出现。在这里，我们鉴定和量化转移的雄性射精蛋白质组和雌性生殖蛋白质组使用三种阳痿组。我们鉴定了200多种雄性射精蛋白和2000多种雌性生殖道蛋白，其中许多蛋白在物种之间表现出不同的丰度，包括雌性特异性丝氨酸型内多肽酶。
Reproductive traits often evolve rapidly between species. Understanding the causes and consequences of this rapid divergence requires characterization of female and male reproductive proteins and their effect on fertilization success. Species in the Drosophila virilis clade exhibit rampant interspecific reproductive incompatibilities, making them ideal for studies on diversification of reproductive proteins and their role in speciation. Importantly, the role of intraejaculate protein abundance and allocation in interspecific divergence is poorly understood. Here, we identify and quantify the transferred male ejaculate proteome using multiplexed isobaric labeling of the lower female reproductive tract before and immediately after mating using three species of the virilis group. We identified over 200 putative male ejaculate proteins, many of which show differential abundance between species, suggesting that males transfer a species-specific allocation of seminal fluid proteins during copulation. We also identified over 2000 female reproductive proteins, which contain female-specific serine-type endopeptidases that showed differential abundance between species and elevated rates of molecular evolution, similar to that of some male seminal fluid proteins. Our findings suggest that reproductive protein divergence can also manifest in terms of species-specific protein abundance patterns. We simultaneously characterize the male and female reproductive proteome. We identified >200 ejaculate proteins and >2000 female reproductive tract proteins. Many proteins show species-specific abundances. Differentially abundant proteins include many belonging to multicopy gene families. Female serine-type endopeptidases show differential abundance and evolve quickly. Molecular interactions between the sexes are critical for fertilization success. However, coevolution of interacting reproductive traits can lead to biochemical mismatches between populations and the emergence of postmating prezygotic isolation. Here, we identify and quantify the transferred male ejaculate proteome and female reproductive proteome using three species of the virilis group. We identified over 200 male ejaculate proteins and over 2000 female reproductive tract proteins, many of which show differential abundance between species, including female-specific serine-type endopeptidases.
登录
查看更多内容
影响因子：
5.8
作者：
Craig, R;Beavis, RC
通讯作者：
Beavis, RC
影响因子：
3.3
作者：
Avila, Frank W.;Cohen, Allie B.;Wolfner, Mariana F.
通讯作者：
Wolfner, Mariana F.
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y
影响因子：
2.6
作者：
Ahmed-Braimah, Yasir H.
通讯作者：
Ahmed-Braimah, Yasir H.
DOI：
10.1155/2012/285468
发表时间：
2012-01-01
期刊：
International journal of evolutionary biology
影响因子：
--
作者：
Ahmed-Braimah, Yasir H;McAllister, Bryant F
通讯作者：
McAllister, Bryant F