The potential for salmon fibrin and thrombin to mitigate pain subsequent to cervical nerve root injury.
The potential for salmon fibrin and thrombin to mitigate pain subsequent to cervical nerve root injury.
复制标题
鲑鱼纤维蛋白和凝血酶有可能减轻颈神经根损伤后的疼痛。
DOI：
10.1016/j.biomaterials.2011.09.021
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发表时间：
2011
期刊：
影响因子：
14
通讯作者：
Winkelstein,BethA
中科院分区：
文献类型：
--
作者：
Weisshaar,ChristineL;Winer,JessamineP;Guarino,BenjaminB;Janmey,PaulA;Winkelstein,BethA
神经根压迫是神经根病的常见原因，并引起持续性疼痛。哺乳动物纤维蛋白在临床上用作凝血剂，但存在各种风险。鱼纤维蛋白是一种潜在的神经损伤治疗的生物材料，因为它促进神经突起的生长，是无毒的，并在较低的温度下容易凝结。本研究在神经根压迫后给予鲑鱼纤维蛋白和凝血酶，并在大鼠疼痛模型中测量行为敏感性和神经胶质活化。与单独损伤相比，纤维蛋白酶和凝血酶均显著降低机械性异常性疼痛（p < 0.02）。用纤维蛋白进行的疼痛压迫表现出异常性疼痛，其在任何一天使用2g细丝刺激时与假手术没有不同;异常性疼痛仅在第1天和第3天使用4g细丝时与假手术显著不同（p < 0.043）。到第5天，纤维蛋白治疗的反应降低至假手术水平。在任何时间点，凝血酶治疗压迫后的异常性疼痛与假手术相比均无变化。在神经根和脊髓小胶质细胞活化的巨噬细胞浸润，只有轻微修改鲑鱼治疗。脊髓星形胶质细胞的表达在纤维蛋白的作用下显著降低（p < 0.0001），但在凝血酶治疗的损伤反应中没有变化。结果表明，鲑鱼纤维蛋白和凝血酶可能是合适的生物材料，以减轻疼痛。
Nerve root compression is a common cause of radiculopathy and induces persistent pain. Mammalian fibrin is used clinically as a coagulant but presents a variety of risks. Fish fibrin is a potential biomaterial for neural injury treatment because it promotes neurite outgrowth, is non-toxic, and clots readily at lower temperatures. This study administered salmon fibrin and thrombin following nerve root compression and measured behavioral sensitivity and glial activation in a rat pain model. Fibrin and thrombin each significantly reduced mechanical allodynia compared to injury alone (p < 0.02). Painful compression with fibrin exhibited allodynia that was not different from sham for any day using stimulation by a 2 g filament; allodynia was only significantly different (p < 0.043) from sham using the 4 g filament on days 1 and 3. By day 5, responses for fibrin treatment decreased to sham levels. Allodynia following compression with thrombin treatment were unchanged from sham at any time point. Macrophage infiltration at the nerve root and spinal microglial activation were only mildly modified by salmon treatments. Spinal astrocytic expression decreased significantly with fibrin (p < 0.0001) but was unchanged from injury responses for thrombin treatment. Results suggest that salmon fibrin and thrombin may be suitable biomaterials to mitigate pain.
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影响因子：
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DOI：
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发表时间：
1998
期刊：
Journal of Toxicology-cutaneous and Ocular Toxicology
影响因子：
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发表时间：
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Arch Phys Med Rehabil 86
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