Process of energy conservation in the extremely haloalkaliphilic methyl‐reducing methanogen Methanonatronarchaeum thermophilum
Process of energy conservation in the extremely haloalkaliphilic methyl‐reducing methanogen Methanonatronarchaeum thermophilum
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极端卤碱甲基还原产甲烷菌Methanonatronarchaeum thermophilum的能量守恒过程
DOI：
10.1111/febs.16165
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Deppenmeier U
中科院分区：
文献类型：
--
作者：
Steiniger F;Sorokin DY;Deppenmeier U
嗜热产甲烷古菌（Methanonatronarchaeium thermophilum）是一种极端嗜盐碱和中度嗜热的古菌，属于嗜盐菌门新的甲烷古菌纲（Methanonatronarchaeia）。关于甲烷古菌纲成员的生理和生物化学知识仍然有限。已知嗜热分枝杆菌进行氢或甲酸盐依赖性甲基还原产甲烷。在这里，我们表明，该生物体能够在所有测试的C1-甲基化底物（甲醇，三甲胺，二甲胺，一甲胺）与甲酸盐或分子氢的组合中生长。中间体（二甲胺或/和一甲胺）在介质中的临时积累发生在消耗的三甲胺或二甲胺。嗜热分枝杆菌的能量守恒依赖于由氢化酶（VhoGAC）、甲酸脱氢酶（FdhGHI）和异二硫键还原酶（HdrDE）组成的呼吸链，这些酶能很好地适应自然环境中的苛刻理化条件。实验揭示了膜中存在两种节能氧化还原酶系统的变体。其中包括H2：杂二硫化物氧化还原酶系统，其已经在甲烷氧化还原酶物种中描述，以及新的甲酸盐：杂二硫化物氧化还原酶系统。后者的电子传递链，这是第一次被实验证明，区别于所有其他已知的产甲烷古菌的有机体，并代表了类甲烷古菌的独特功能。用2-羟基吩嗪和抑制剂氯化二苯碘铵进行的实验表明，甲吩嗪样辅因子可能在氢化酶/甲酸脱氢酶和杂二硫还原酶之间起电子载体的作用。
The recently isolated methanogenMethanonatronarchaeum thermophilumis an extremely haloalkaliphilic and moderately thermophilic archaeon and belongs to the novel class Methanonatronarchaeia in the phylum Halobacteriota. The knowledge about the physiology and biochemistry of members of the class Methanonatronarchaeia is still limited. It is known thatM. thermophilumperforms hydrogen or formate‐dependent methyl‐reducing methanogenesis. Here, we show that the organism was able to grow on all tested C1‐methylated substrates (methanol, trimethylamine, dimethylamine, monomethylamine) in combination with formate or molecular hydrogen. A temporary accumulation of intermediates (dimethylamine or/and monomethylamine) in the medium occurred during the consumption of trimethylamine or dimethylamine. The energy conservation ofM. thermophilumwas dependent on a respiratory chain consisting of a hydrogenase (VhoGAC), a formate dehydrogenase (FdhGHI), and a heterodisulfide reductase (HdrDE) that were well adapted to the harsh physicochemical conditions in the natural habitat. The experiments revealed the presence of two variants of energy‐conserving oxidoreductase systems in the membrane. These included the H2: heterodisulfide oxidoreductase system, which has already been described inMethanosarcinaspecies, as well as the novel formate: heterodisulfide oxidoreductase system. The latter electron transport chain, which was experimentally proven for the first time, distinguishes the organism from all other known methanogenic archaea and represents a unique feature of the class Methanonatronarchaeia. Experiments with 2‐hydroxyphenazine and the inhibitor diphenyleneiodonium chloride indicated that a methanophenazine‐like cofactor might function as an electron carrier between the hydrogenase/ formate dehydrogenase and the heterodisulfide reductase.
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DOI：
10.1046/j.1432-1327.1999.00017.x
发表时间：
1999-01-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
Brodersen, J;Bäumer, S;Deppenmeier, U
通讯作者：
Deppenmeier, U
随机诱变鉴定了产甲烷古菌 Methanococcusmaripaludis 依赖甲酸盐生长的相关因素
DOI：
--
发表时间：
2013
期刊：
Zeitschrift für Induktive Abstammungs- und Vererbungslehre
影响因子：
--
作者：
C. Sattler;S. Wolf;Julia Fersch;S. Goetz;M. Rother
通讯作者：
M. Rother
二亚苯基碘鎓抑制线粒体 NADH-泛醌氧化还原酶（复合物 I）中铁硫簇的还原。
影响因子：
4.8
作者：
A. Majander;M. Finel;M. Wikström
通讯作者：
M. Wikström
甲烷微生物古菌中依赖甲酸盐的异二硫键还原
影响因子：
4.4
作者：
M. A. Abdul Halim;L. Day;K. Costa
通讯作者：
K. Costa
真养产碱菌膜结合氢化酶的抗原决定簇暴露于周质
影响因子：
3.2
作者：
K. Eismann;K. Mlejnek;D. Zipprich;M. Hoppert;H. Gerberding;F. Mayer
通讯作者：
F. Mayer