Adenosine A2A receptor stimulation increases release of acetylcholine from rat hippocampus but not striatum, and does not affect catecholamine release
Adenosine A2A receptor stimulation increases release of acetylcholine from rat hippocampus but not striatum, and does not affect catecholamine release
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腺苷 A2A 受体刺激会增加大鼠海马而非纹状体乙酰胆碱的释放，并且不影响儿茶酚胺的释放
DOI：
10.1007/pl00004917
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
B. Fredholm
中科院分区：
文献类型：
--
作者：
Shaoyu Jin;B. Fredholm
用[3H]多巴胺（DA）{或[3H]去甲肾上腺素（NA）}和[14C]胆碱预孵育大鼠纹状体和海马切片，连续灌注并电刺激。2-氯腺苷（2-CADO 0.001 ~ 100 μM）是一种非选择性腺苷受体激动剂，对纹状体片电诱发DA和乙酰胆碱（ACh）释放以及海马片电诱发NA和ACh释放产生浓度依赖性抑制。8-环戊基-1,3-二丙基黄嘌呤（DPCPX 3,30和200 nM）是一种选择性腺苷A1受体拮抗剂，引起2-CADO浓度响应曲线的浓度依赖，平行，向右移动，符合竞争性拮抗剂。pA2值在8.4 ~ 8.8之间。在海马释放乙酰胆碱的情况下，在DPCPX存在的情况下，低浓度的2-CADO的放射性释放是否增加。海马区的刺激可被选择性腺苷A2A受体拮抗剂KF 17837阻断。KF 17837 （0.1 ~ 100 μM）本身对海马片电诱发NA释放无影响，但可降低电诱发ACh释放。这种抑制作用被1 μM的DPCPX抵消。这些结果表明，在所使用的条件下，纹状体中DA的释放、海马中NA的释放以及纹状体中ACh的释放受腺苷A1而非腺苷A2A受体的调节。相比之下，海马的乙酰胆碱释放受到抑制释放的腺苷A1受体和刺激释放的腺苷A2A受体的强直调节。
Abstract Rat striatal and hippocampal slices, preincubated with [3H] dopamine (DA) {or [3H] noradrenaline (NA)} and [14C] choline, were superfused continuously and stimulated electrically. 2-chloroadenosine (2-CADO 0.001–100 μM), a non-selective adenosine receptor agonist, produced a concentration-dependent inhibition of the electrically evoked DA and acetylcholine (ACh) release from the striatal slices and of the electrically evoked NA and ACh release from the hippocampal slices. 8-cyclopentyl-1,3-dipropylxanthine (DPCPX 3, 30 and 200 nM), a selective adenosine A1 receptor antagonist, caused a concentration-dependent, parallel, rightward shift of the 2-CADO concentration-response curve, consistent with competitive antagonism. The pA2 values ranged between 8.4 and 8.8. In the case of ACh release from the hippocampus, but in no other case, was there an increase in release of radioactivity at low concentrations of 2-CADO in the presence of DPCPX. The stimulation in the hippocampus could be blocked by a selective adenosine A2A receptor antagonist KF 17837. By itself KF 17837 (0.1–100 μM) had no effect on electrically evoked NA release from hippocampal slices, but decreased electrically evoked ACh release. This inhibition was counteracted by DPCPX (1 μM).These results show that, under the conditions used, DA release in the striatum, and NA release in the hippocampus, as well as ACh release from the striatum are regulated by adenosine A1 but not by adenosine A2A receptors. By contrast, ACh release from the hippocampus is tonically regulated both by adenosine A1 receptors, which inhibit release, and by adenosine A2A receptors which stimulate release.
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DOI：
--
发表时间：
2005
期刊：
--
影响因子：
--
作者：
Bertil;-B.;Fredholm;Maria;-P.;Abbracchio;Geoffrey;Burnstock;John;-W.;Daly;-T.;Kendall
通讯作者：
Bertil;-B.;Fredholm;Maria;-P.;Abbracchio;Geoffrey;Burnstock;John;-W.;Daly;-T.;Kendall
KF17837 是一种体内 A2 腺苷受体拮抗剂。
DOI：
--
发表时间：
1993
期刊：
The Journal of pharmacology and experimental therapeutics
影响因子：
--
作者：
Jackson,EK;Herzer,WA;Suzuki,F
通讯作者：
Suzuki,F
影响因子：
3.6
作者：
M. Abbracchio;Roberta Brambilla;S. Ceruti;Hea Ok Kim;D. V. Lubitz;Kenneth A. Jacobson;Flaminio Cattabeni
通讯作者：
M. Abbracchio;Roberta Brambilla;S. Ceruti;Hea Ok Kim;D. V. Lubitz;Kenneth A. Jacobson;Flaminio Cattabeni
选择性腺苷 A2 受体激动剂 CGS 21680 对大鼠海马和纹状体体外电生理学、cAMP 形成和多巴胺释放的影响。
DOI：
--
发表时间：
1990
期刊：
The Journal of pharmacology and experimental therapeutics
影响因子：
--
作者：
Lupica,CR;Cass,WA;Zahniser,NR;Dunwiddie,TV
通讯作者：
Dunwiddie,TV
影响因子：
56.9
作者：
RAINNIE, DG;GRUNZE, HCR;GREENE, RW
通讯作者：
GREENE, RW