Overexpression of MdATG8i improves water use efficiency in transgenic apple by modulating photosynthesis, osmotic balance, and autophagic activity under moderate water deficit.
Overexpression of MdATG8i improves water use efficiency in transgenic apple by modulating photosynthesis, osmotic balance, and autophagic activity under moderate water deficit.
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DOI：
10.1038/s41438-021-00521-2
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发表时间：
2021-04-01
影响因子：
8.7
通讯作者：
Ma F
中科院分区：
文献类型：
--
作者：
Jia X;Mao K;Wang P;Wang Y;Jia X;Huo L;Sun X;Che R;Gong X;Ma F
水分亏缺是中国苹果主产区黄土高原苹果（Malus domestica）生产的主要限制因素之一。与水分利用效率（WUE）调控相关的基因鉴定是作物育种计划的一个重要方面。自噬作为一种保守的真核生物降解和再循环机制，已被报道参与各种应激反应。然而，自噬与WUE调节之间的关系尚未探讨。我们已经证明苹果中一个关键的自噬蛋白MdATG8i在提高耐盐性中起作用。本研究探讨了其在长期中等干旱胁迫下的生物学功能。结果表明，mdatg8i过表达（MdATG8i-OE）苹果植株在长期中度干旱条件下的水分利用效率高于野生型（WT）植株。植物水分利用效率可以通过提高光合效率来提高。渗透调节在植物抗逆性和适应性中起着至关重要的作用。在长期干旱条件下，MdATG8i-OE植株的光合能力、糖和氨基酸积累量均高于WT植株。OE植物光合能力的增强可能与它们保持最佳气孔孔径、有组织的叶绿体和较强的抗氧化活性有关。MdATG8i过表达也促进了自噬活性，这可能与上述变化有关。综上所述，我们的研究结果表明，MdATG8i-OE苹果品系在长期中等干旱条件下表现出比WT更高的水分利用效率，因为它们保持了强劲的光合作用、有效的渗透调节过程和强大的自噬活性。
Water deficit is one of the major limiting factors for apple (Malus domestica) production on the Loess Plateau, a major apple cultivation area in China. The identification of genes related to the regulation of water use efficiency (WUE) is a crucial aspect of crop breeding programs. As a conserved degradation and recycling mechanism in eukaryotes, autophagy has been reported to participate in various stress responses. However, the relationship between autophagy and WUE regulation has not been explored. We have shown that a crucial autophagy protein in apple, MdATG8i, plays a role in improving salt tolerance. Here, we explored its biological function in response to long-term moderate drought stress. The results showed that MdATG8i-overexpressing (MdATG8i-OE) apple plants exhibited higher WUE than wild-type (WT) plants under long-term moderate drought conditions. Plant WUE can be increased by improving photosynthetic efficiency. Osmoregulation plays a critical role in plant stress resistance and adaptation. Under long-term drought conditions, the photosynthetic capacity and accumulation of sugar and amino acids were higher in MdATG8i-OE plants than in WT plants. The increased photosynthetic capacity in the OE plants could be attributed to their ability to maintain optimal stomatal aperture, organized chloroplasts, and strong antioxidant activity. MdATG8i overexpression also promoted autophagic activity, which was likely related to the changes described above. In summary, our results demonstrate that MdATG8i-OE apple lines exhibited higher WUE than WT under long-term moderate drought conditions because they maintained robust photosynthesis, effective osmotic adjustment processes, and strong autophagic activity.
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DOI：
10.1073/pnas.191389398
发表时间：
2001-09-25
影响因子：
11.1
作者：
Gaxiola, RA;Li, JS;Fink, GR
通讯作者：
Fink, GR
影响因子：
6.5
作者：
Du, Yanli;Zhao, Qiang;Xie, Futi
通讯作者：
Xie, Futi
影响因子：
9.4
作者：
Guiboileau, Anne;Avila-Ospina, Liliana;Masclaux-Daubresse, Celine
通讯作者：
Masclaux-Daubresse, Celine
影响因子：
3.9
作者：
HEATH, RL;PACKER, L
通讯作者：
PACKER, L
影响因子：
2.3
作者：
EHDAIE, B
通讯作者：
EHDAIE, B