Light-Dependent Degradation of PIF3 by SCF(EBF1/2) Promotes a Photomorphogenic Response in Arabidopsis.
Light-Dependent Degradation of PIF3 by SCF(EBF1/2) Promotes a Photomorphogenic Response in Arabidopsis.
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SCF（EBF1/2）对PIF3的光依赖性降解促进了拟南芥中的光性反应。
DOI：
10.1016/j.cub.2017.06.062
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发表时间：
2017-08-21
期刊：
影响因子：
--
通讯作者：
Wei N
中科院分区：
文献类型：
--
作者：
Dong J;Ni W;Yu R;Deng XW;Chen H;Wei N
植物幼苗从黑暗中进入光环境经历光形态建成，这使得具有优化的形态和生理的自养生长成为可能。在这一转变过程中，植物必须迅速清除在黑暗中积累的光形态发生抑制因子。其中之一是植物色素相互作用因子3（PIF 3），一种促进下胚轴生长的关键转录因子。在这里，我们报告说，为了响应光敏色素光感受器的光激活，EIN 3结合F盒蛋白质（EBF）、EBF 1和EBF 2以依赖于光诱导的PIF 3磷酸化的方式介导PIF 3蛋白降解。虽然PIF 3独立于光结合EBF，但PIF 3-EBF向核心SCF支架的募集通过光信号或PIF 3磷酸化来促进。我们还发现，先前鉴定的光响应BRIC-A-BRAKE/TRAMTRACK/BROAD（LRB）E3泛素连接酶主要在强光条件下靶向光敏色素B（phyB）和PIF 3，而EBF 1/2在宽范围的光强度下强烈靶向PIF 3降解而不影响phyB的丰度。遗传和分子数据均支持SCFEBF 1/2在幼苗发育过程中作为光形态发生E3的功能。
Plant seedlings emerging from darkness into the light environment undergo photomorphogenesis, which enables autotrophic growth with optimized morphology and physiology. During this transition, plants must rapidly remove photomorphogenic repressors accumulated in the dark. Among them is PHYTOCHROME-INTERACTING FACTOR 3 (PIF3), a key transcription factor promoting hypocotyl growth. Here we report that, in response to light activation of phytochrome photoreceptors, EIN3-BINDING F BOX PROTEINs (EBFs), EBF1 and EBF2, mediate PIF3 protein degradation in a manner dependent on light-induced phosphorylation of PIF3. While PIF3 binds EBFs independently of light, the recruitment of PIF3-EBFs to the core SCF scaffold is facilitated by light signals or PIF3 phosphorylation. We also found that previously identified LIGHT-RESPONSE BRIC-A-BRACK/TRAMTRACK/BROAD (LRB) E3 ubiquitin ligases target phytochrome B (phyB) and PIF3 primarily under high light conditions, whereas EBF1/2 vigorously target PIF3 degradation under wide ranges of light intensity without affecting the abundance of phyB. Both genetic and molecular data support that SCFEBF1/2 function as the photomorphogenic E3s during seedling development.
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影响因子：
16.6
作者：
Ni W;Xu SL;González-Grandío E;Chalkley RJ;Huhmer AFR;Burlingame AL;Wang ZY;Quail PH
通讯作者：
Quail PH
DOI：
10.1126/science.1250778
发表时间：
2014-06-06
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Ni W;Xu SL;Tepperman JM;Stanley DJ;Maltby DA;Gross JD;Burlingame AL;Wang ZY;Quail PH
通讯作者：
Quail PH
影响因子：
56.9
作者：
Martínez-García, JF;Huq, E;Quail, PH
通讯作者：
Quail, PH
影响因子：
6.9
作者：
Monte, Elena;Al-Sady, Bassem;Quail, Peter H.
通讯作者：
Quail, Peter H.
影响因子：
64.8
作者：
Ni, M;Tepperman, JM;Quail, PH
通讯作者：
Quail, PH