Bacillus subtilis RecO and SsbA are crucial for RecA-mediated recombinational DNA repair.
Bacillus subtilis RecO and SsbA are crucial for RecA-mediated recombinational DNA repair.
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DOI：
10.1093/nar/gkv545
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发表时间：
2015-07-13
影响因子：
14.9
通讯作者：
Alonso JC
中科院分区：
文献类型：
--
作者：
Carrasco B;Yadav T;Serrano E;Alonso JC
遗传数据显示，枯草芽孢杆菌RecO和其中一种末端加工途径（AddAB或RecJ）的缺失使细胞对DNA损伤剂的敏感性与无recA一样，这表明这两种末端切除途径都需要RecO进行重组。RecA，以rATP·Mg2+结合形式（RecA·ATP），对催化线性双链DNA （ds）和裸互补环状单链DNA （ss）之间的DNA重组无活性。我们发现RecA·ATP不能在SsbA·ssDNA或SsbB·ssDNA复合物上成核和/或聚合。RecA·ATP在RecO·ssDNA·SsbA复合物上成核和聚合的效率高于RecO·ssDNA·SsbB复合物。限制SsbA浓度足以刺激RecA·ATP在RecO·ssDNA·SsbB复合物上的组装。RecO和SsbA是“激活”RecA·ATP催化DNA链交换的必要和充分条件，而AddAB复合体、RecO单独或与SsbB协同作用是不够的。AddAB、RecO和SsbA的存在仍然是RecA·atp介导的高效三链交换重组所必需的。基于遗传和生化数据，我们提出SsbA和RecO（或体内的SsbA、RecO和RecR）在RecA激活AddAB和RecJ-RecQ （RecS）重组修复途径中都是至关重要的。
Genetic data have revealed that the absence of Bacillus subtilis RecO and one of the end-processing avenues (AddAB or RecJ) renders cells as sensitive to DNA damaging agents as the null recA, suggesting that both end-resection pathways require RecO for recombination. RecA, in the rATP·Mg2+ bound form (RecA·ATP), is inactive to catalyze DNA recombination between linear double-stranded (ds) DNA and naked complementary circular single-stranded (ss) DNA. We showed that RecA·ATP could not nucleate and/or polymerize on SsbA·ssDNA or SsbB·ssDNA complexes. RecA·ATP nucleates and polymerizes on RecO·ssDNA·SsbA complexes more efficiently than on RecO·ssDNA·SsbB complexes. Limiting SsbA concentrations were sufficient to stimulate RecA·ATP assembly on the RecO·ssDNA·SsbB complexes. RecO and SsbA are necessary and sufficient to ‘activate’ RecA·ATP to catalyze DNA strand exchange, whereas the AddAB complex, RecO alone or in concert with SsbB was not sufficient. In presence of AddAB, RecO and SsbA are still necessary for efficient RecA·ATP-mediated three-strand exchange recombination. Based on genetic and biochemical data, we proposed that SsbA and RecO (or SsbA, RecO and RecR in vivo) are crucial for RecA activation for both, AddAB and RecJ–RecQ (RecS) recombinational repair pathways.
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影响因子：
4.5
作者：
Attaiech L;Olivier A;Mortier-Barrière I;Soulet AL;Granadel C;Martin B;Polard P;Claverys JP
通讯作者：
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影响因子：
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作者：
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作者：
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通讯作者：
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