Disease Modeling Using 3D Organoids Derived from Human Induced Pluripotent Stem Cells.
Disease Modeling Using 3D Organoids Derived from Human Induced Pluripotent Stem Cells.
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使用来自人类诱导多能干细胞的3D类器官进行疾病建模。
DOI：
10.3390/ijms19040936
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发表时间：
2018-03-21
影响因子：
5.6
通讯作者：
Soh BS
中科院分区：
文献类型：
--
作者：
Ho BX;Pek NMQ;Soh BS
对人类诱导多能干细胞（hiPSC）衍生的类器官培养的兴趣日益浓厚，这源于对定向多谱系分化和模仿器官发生的形态发生过程的各种组合的操纵。类器官是由多种细胞类型组成的三维（3D）结构，在体外自我组织以概括胚胎和组织发育。该模型已被证明优于传统的二维（2D）细胞培养方法，在镜像功能、结构和体内组织的几何特征方面。本综述旨在强调3D类器官技术的最新进展，用于复杂遗传疾病、癌症、宿主-微生物相互作用的建模，以及在转化和个性化医学中的可能应用，其中类器官培养用于通过高通量药物筛选发现早期疾病检测的诊断性生物标志物。此外，本文还旨在讨论利用类器官进行疾病建模的优点和缺点。总之，使用hipsc衍生的类器官研究人类疾病可能更好地说明所涉及的过程，因为类器官中存在的结构和微环境相似，这也允许在体外适当地重现药物反应。
The rising interest in human induced pluripotent stem cell (hiPSC)-derived organoid culture has stemmed from the manipulation of various combinations of directed multi-lineage differentiation and morphogenetic processes that mimic organogenesis. Organoids are three-dimensional (3D) structures that are comprised of multiple cell types, self-organized to recapitulate embryonic and tissue development in vitro. This model has been shown to be superior to conventional two-dimensional (2D) cell culture methods in mirroring functionality, architecture, and geometric features of tissues seen in vivo. This review serves to highlight recent advances in the 3D organoid technology for use in modeling complex hereditary diseases, cancer, host–microbe interactions, and possible use in translational and personalized medicine where organoid cultures were used to uncover diagnostic biomarkers for early disease detection via high throughput pharmaceutical screening. In addition, this review also aims to discuss the advantages and shortcomings of utilizing organoids in disease modeling. In summary, studying human diseases using hiPSC-derived organoids may better illustrate the processes involved due to similarities in the architecture and microenvironment present in an organoid, which also allows drug responses to be properly recapitulated in vitro.
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人类肺发育和疾病的三维模型来自多能干细胞。
影响因子：
21.3
作者：
Chen YW;Huang SX;de Carvalho ALRT;Ho SH;Islam MN;Volpi S;Notarangelo LD;Ciancanelli M;Casanova JL;Bhattacharya J;Liang AF;Palermo LM;Porotto M;Moscona A;Snoeck HW
通讯作者：
Snoeck HW
影响因子：
64.5
作者：
Gao D;Vela I;Sboner A;Iaquinta PJ;Karthaus WR;Gopalan A;Dowling C;Wanjala JN;Undvall EA;Arora VK;Wongvipat J;Kossai M;Ramazanoglu S;Barboza LP;Di W;Cao Z;Zhang QF;Sirota I;Ran L;MacDonald TY;Beltran H;Mosquera JM;Touijer KA;Scardino PT;Laudone VP;Curtis KR;Rathkopf DE;Morris MJ;Danila DC;Slovin SF;Solomon SB;Eastham JA;Chi P;Carver B;Rubin MA;Scher HI;Clevers H;Sawyers CL;Chen Y
通讯作者：
Chen Y
影响因子：
5.9
作者：
DiStefano T;Chen HY;Panebianco C;Kaya KD;Brooks MJ;Gieser L;Morgan NY;Pohida T;Swaroop A
通讯作者：
Swaroop A
影响因子：
9.9
作者：
Casanova, MF;Buxhoeveden, DP;Roy, E
通讯作者：
Roy, E
使用CRISPR/CAS9编辑HIPSC的基因组：疾病模型。
DOI：
10.1007/s00335-017-9684-9
发表时间：
2017-08
期刊：
Mammalian genome : official journal of the International Mammalian Genome Society
影响因子：
--
作者：
Bassett AR
通讯作者：
Bassett AR