Vertical sleeve gastrectomy reduces hepatic steatosis while increasing serum bile acids in a weight-loss-independent manner.
Vertical sleeve gastrectomy reduces hepatic steatosis while increasing serum bile acids in a weight-loss-independent manner.
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垂直套筒胃切除术减少肝脂肪变性，同时以减轻体重的方式增加血清胆汁酸。
DOI：
10.1002/oby.20548
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发表时间：
2014-02
期刊：
影响因子：
--
通讯作者：
Kohli R
中科院分区：
文献类型：
--
作者：
Myronovych A;Kirby M;Ryan KK;Zhang W;Jha P;Setchell KD;Dexheimer PJ;Aronow B;Seeley RJ;Kohli R
我们的目的是探讨胆汁酸在垂直袖状胃切除术(VSG)后减轻肝脏脂肪变性中的作用。将高脂饮食(HFD)诱导的肥胖C57BL/6小鼠随机分为假手术组(Sham)、假手术组(Sham)、假手术+VSG组(Sham-PF)和非手术对照组(Naive)。所有小鼠在处死前一直服用HFD。术后观察小鼠体重、体成分、代谢参数、血清胆汁酸水平和成分。进一步通过RNAseq和RT-PCR分析肝脏基因的表达。VSG和Sham-PF小鼠在手术后体重下降相同，而VSG小鼠的肝脏甘油三酯含量在牺牲时最低。VSG组小鼠的血清胆汁酸水平升高，与最大体重减轻呈正相关。VSG组血清胆汁成分中胆酸和牛磺熊去氧胆酸升高。VSG小鼠的这些胆汁酸组成的变化解释了观察到的肝脏造脂和胆汁酸合成基因的下调。与单纯限制热量摄入相比，肥胖小鼠的VSG可导致更大程度的肝脏脂肪变性减轻。VSG手术增加了与术后体重减轻相关的血清胆汁酸，并改变了血清胆汁成分，这可能解释了抑制肝脏脂肪生成的基因。
Our objective was to investigate the role of bile acids in hepatic steatosis reduction after vertical sleeve gastrectomy (VSG). High fat diet (HFD) induced obese C57Bl/6 mice were randomized to: VSG, Sham operation (Sham), Sham operation with pair feeding to VSG (Sham-PF), or non-surgical controls (Naïve). All mice were on HFD until sacrifice. Mice were observed post-surgery and data for body weight, body composition, metabolic parameters, serum bile acid level and composition were collected. Further hepatic gene expression by RNAseq and RT-PCR analysis was assessed. VSG and Sham-PF mice lost equal weight post-surgery while VSG mice had the lowest hepatic triglyceride content at sacrifice. The VSG mice had elevated serum bile acid levels that positively correlated with maximal weight loss. Serum bile composition in the VSG group had increased cholic and tauroursodeoxycholic acid. These bile acid composition changes in VSG mice explained observed downregulation of hepatic lipogenic and bile acid synthetic genes. VSG in obese mice results in greater hepatic steatosis reduction than seen with caloric restriction alone. VSG surgery increases serum bile acids that correlate with weight lost post-surgery and changes serum bile composition that could explain suppression of hepatic genes responsible for lipogenesis.
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DOI：
10.1038/oby.2009.102
发表时间：
2009-09
期刊：
Obesity (Silver Spring, Md.)
影响因子：
--
作者：
Patti ME;Houten SM;Bianco AC;Bernier R;Larsen PR;Holst JJ;Badman MK;Maratos-Flier E;Mun EC;Pihlajamaki J;Auwerx J;Goldfine AB
通讯作者：
Goldfine AB
影响因子：
29.4
作者：
Stefater MA;Pérez-Tilve D;Chambers AP;Wilson-Pérez HE;Sandoval DA;Berger J;Toure M;Tschöp M;Woods SC;Seeley RJ
通讯作者：
Seeley RJ
DOI：
10.1016/j.soard.2009.08.009
发表时间：
2010-07
期刊：
Surgery for obesity and related diseases : official journal of the American Society for Bariatric Surgery
影响因子：
--
作者：
Shah SS;Todkar JS;Shah PS;Cummings DE
通讯作者：
Cummings DE
影响因子：
4.9
作者：
Ahmad, N. N.;Pfalzer, A.;Kaplan, L. M.
通讯作者：
Kaplan, L. M.
影响因子：
2.4
作者：
Giorgio V;Prono F;Graziano F;Nobili V
通讯作者：
Nobili V