Dicer-dependent biogenesis of small RNAs derived from 7SL RNA.
Dicer-dependent biogenesis of small RNAs derived from 7SL RNA.
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DOI：
10.1371/journal.pone.0040705
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Tang KF
中科院分区：
文献类型：
--
作者：
Ren YF;Li G;Wu J;Xue YF;Song YJ;Lv L;Zhang XJ;Tang KF
已有研究表明，人视网膜色素上皮细胞中Alu RNAs的表达减少导致Alu RNAs的积聚，Disher可能将内源性Sine/B1 RNAs(相当于灵长类Alu RNAs)加工成小干扰RNA(SiRNAs)。在本研究中，我们旨在探讨DICER是否能够处理Alu RNA及其共同的祖先7SL RNA。利用Solexa测序技术，我们发现在HepG2.2.15细胞中，Alu衍生的小RNA占细胞小RNA总数的0.6%，并且当Dier被击倒时，其丰度降低。然而，Alu衍生的小RNA显示出不同于经典Dice加工产品的miRNAs和siRNAs的特征。有趣的是，我们发现在对照细胞中，来自7SL RNA的小RNA占细胞总小RNA的3.1%，而在Dice基因敲除细胞中，其丰度下降了约3.4倍。通过Northern blotting验证了7SL RNA衍生的小RNA的依赖于Dice的生物发生。体外切割实验也表明，人工合成的7SL RNA被Disher加工成不同长度的片段。进一步的功能分析表明，7SL RNA衍生的小RNA不像miRNAs那样发挥功能，也不调控7SL RNA的表达。综上所述，目前的研究表明，DICER可以处理7SL RNA，但其生物学意义仍有待阐明。
It has been reported that decreased Dicer expression leads to Alu RNAs accumulation in human retinal pigmented epithelium cells, and Dicer may process the endogenous SINE/B1 RNAs (the rodent equivalent of the primate Alu RNAs) into small interfering RNAs (siRNAs). In this study, we aimed to address whether Dicer can process Alu RNAs and their common ancestor, 7SL RNA. Using Solexa sequencing technology, we showed that Alu-derived small RNAs accounted for 0.6% of the total cellular small RNAs in HepG2.2.15 cells, and the abundance decreased when Dicer was knocked down. However, Alu-derived small RNAs showed different characteristics from miRNAs and siRNAs, the classic Dicer-processed products. Interestingly, we found that small RNAs derived from 7SL RNA accounted for 3.1% of the total cellular small RNAs in the control cells, and the abundance dropped about 3.4 folds in Dicer knockdown cells. Dicer-dependent biogenesis of 7SL RNA-derived small RNAs was validated by northern blotting. In vitro cleavage assay using the recombinant human Dicer protein also showed that synthetic 7SL RNA was processed by Dicer into fragments of different lengths. Further functional analysis suggested that 7SL RNA-derived small RNAs do not function like miRNAs, neither do they regulate the expression of 7SL RNA. In conclusion, the current study demonstrated that Dicer can process 7SL RNA, however, the biological significance remains to be elucidated.
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影响因子：
10.5
作者：
Babiarz, Joshua E.;Ruby, J. Graham;Blelloch, Robert
通讯作者：
Blelloch, Robert
影响因子：
4.5
作者：
Ritchie, William;Legendre, Matthieu;Gautheret, Daniel
通讯作者：
Gautheret, Daniel
影响因子：
4.5
作者：
Pillai, RS;Artus, CG;Filipowicz, W
通讯作者：
Filipowicz, W
影响因子：
15.9
作者：
Liu, Xi;Sempere, Lorenzo F.;Dmitrovsky, Ethan
通讯作者：
Dmitrovsky, Ethan
DOI：
10.1038/nrg2640
发表时间：
2009-10
期刊：
Nature reviews. Genetics
影响因子：
--
作者：
通讯作者：
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